3种益生菌对抗生素相关性腹泻小鼠肠道乳酸杆菌数的影响

Effect of three probiotics on the number of intestinal lactobacilli in mice with antibiotic-associated diarrhea

目的 观察双歧杆菌活菌、酵母片及乳酸菌素片对抗生素相关性腹泻(ADD)小鼠肠道乳酸杆菌数的影响。方法 40只1月龄小鼠用抗生素灌胃制作ADD模型,随机分为模型组、双歧杆菌活菌组、酵母片组、乳酸菌素片组,另取10只小鼠作为空白组;空白组与模型组给予蒸馏水灌胃,双歧杆菌活菌组、酵母片组、乳酸菌素片组分别给予0.175 g/kg双歧杆菌活菌胶囊、0.6 g/kg酵母片、0.9 g/kg乳酸菌素片灌胃,连用9 d;采用实时荧光定量PCR检测小鼠盲肠内乳酸杆菌含量。结果 与空白组比较,模型组盲肠内乳酸杆菌群含量明显减少(P<0.01);经过2~8 d治疗,双歧杆菌活菌组、酵母片组及乳酸菌素片组盲肠内乳酸杆菌群含量明显高于模型组(P<0.01),以乳酸菌素片组更为显著(P<0.01)。结论 双歧杆菌活菌、酵母片及乳酸菌素片对ADD小鼠肠道乳酸杆菌数具有调理作用,其中乳酸菌素片效果更好。

Objective To observe the effect of live bifidobacteria, yeast tablets and lactobacillus tablets on the number of intestinal lactobacilli in mice with antibiotic-associated diarrhea(ADD). Methods Forty 1-month-old mice were chosen to establish ADD models by intragastrical antibiotics, and then randomized to model, live bifidobacteria, yeast tablet and lactobacillus tablet groups;10 mice acted as blank group. The distilled water was intragastrically given in blank and model groups, while 0.175 g/kg live bifidobacteria capsules, 0.6 g/kg yeast tablets, and 0.9 g/kg lactobacillus tablets were respectively given for 9 days in live bifidobacteria, yeast tablet and lactobacillus tablet groups. The number of cecal lactobacilli was detected by real-time fluorescence quantitative PCR. Results The number of cecal lactobacilli was lower in model group than in blank group(P<0.01). Compared with model group, the number of cecal lactobacilli was increased in 3 probiotic groups(P<0.01),especially in lactobacillus tablet group(P<0.01). Conclusion The live bifidobacteria, yeast tablet and lactobacillus tablet can regulate the number of intestinal lactobacilli in mice with ADD, with the best of lactobacillus tablet.