黄连解毒汤调控apoE^(-/-)小鼠血脂及低密度脂蛋白亚组分水平的研究

Effect of Huanglian Jiedu Decoction on Regulating Blood Lipids and Low Density Lipoprotein Subfractions in Plasma of apoE^(-/-)Mice

目的观察黄连解毒汤对高脂饮食载脂蛋白E基因敲除(apoE^(-/-))小鼠血脂水平、血脂组分比例和低密度脂蛋白(LDL)亚组分水平的影响。方法24只apoE^(-/-)小鼠随机分为阴性对照组(普通饮食)、模型组(高脂饮食)、中药组[高脂饮食+黄连解毒汤5 g/(kgd)灌胃]、西药组[高脂饮食+阿托伐他汀3 mg/(kgd)灌胃],另设6只C57BL/6小鼠为正常对照组给予普通饮食。4周后,常规生化检测血浆血脂水平;Quantimetrix Lipoprint系统检测血浆脂质组分比例和LDL亚组分水平。结果与正常对照组比较,阴性对照组小鼠血清总胆固醇(TC)、LDL、LDL1~7水平、血浆极低密度脂蛋白(VLDL)、中密度脂蛋白(IDL)和LDL比例升高,高密度脂蛋白(HDL)水平及比例降低(P<0.05,P<0.01);与阴性对照组比较,模型组小鼠TC、LDL、LDL1~7水平、VLDL比例、小而密低密度脂蛋白(sdLDL)/LDL升高(P<0.05,P<0.01);与模型组比较,中药组和西药组小鼠VLDL比例降低(P<0.05,P<0.01),中药组LDL比例升高(P<0.05),中药组LDL7水平降低(P<0.05),西药组小鼠LDL5~7水平降低(P<0.05)。结论高脂饮食可导致apoE^(-/-)小鼠血脂水平升高,sdLDL特别是LDL7水平升高,黄连解毒汤干预可降低LDL7水平。

Objective To observe the effects of Huanglian Jiedu Decoction(HJD)on plasma lipid level,proportion of plasma lipid components and level of low density lipoprotein(LDL)subfractions in high fat diet(HFD)apolipoprotein E gene knockout(apoE^(-/-))mice.Methods Twenty-four apoE^(-/-)mice were randomly divided into negative control group(chow diet),model group(HFD),Chinese medicine group[HFD+HJD 5 g/(kg d)by gavage]and Western medicine group[HFD+atorvastatin 3 mg/(kg d)by gavage],another 6 C57BL/6 mice were set as normal control group and fed chow diet.After 4 weeks,the levels of plasma lipids were detected by routine biochemical method,proportion of plasma lipid components and LDL subfractions level were detected by Quantimetrix Lipoprint system.Results Compared with the normal control group,the levels of total cholesterol(TC),LDL,LDL1~7,the proportion of very low density lipoproteins(VLDL),intermediate density lipoproteins(IDL)and LDL in plasma increased,the level and proportion of high density lipoprotein(HDL)decreased(P<0.05,P<0.01).Compared with the negative control group,the levels of TC,LDL,LDL1~7,the proportion of VLDL and small and dense low density lipoprotein(sd LDL)/LDL in the model group increased(P<0.05,P<0.01).Compared with the model group,the proportion of VLDL in the Chinese medicine group and the Western medicine group decreased(P<0.05,P<0.01),the proportion of LDL in the Chinese medicine group increased(P<0.05),and the level of LDL 7 in the Chinese medicine group decreased(P<0.05),the level of LDL 5~7in Western medicine group decreased(P<0.05).Conclusions HFD could induce hyperlipidemia,increase level of sd LDL,especially LDL7 in apoE^(-/-)mice.HJD could reduce the level of LDL7.