基于基因芯片对癫痫小鼠海马组织miRNA-204表达的研究及生物信息学分析

Study and bioinformatics analysis of miRNA-204 expression in hippocampal tissues of epileptic mice based on gene chip

目的基于基因芯片对癫痫小鼠海马组织miRNA-204表达进行分析研究。方法选取癫痫小鼠海马组织,利用基因芯片技术检测出差异基因,并利用生物信息学对其靶基因进行富集分析和通路分析。结果本实验检测到与癫痫相关差异基因miRNA-204表达下调,预测到相关457个相交集靶基因,富集分析得到27个基因功能(P<0.01)和16个信号通路(P<0.05)。结论通过分析,明确miRNA-204表达下调对癫痫小鼠的影响,并分析出miRNA-204相关通路Wnt,为进一步研究miRNA-204作用于癫痫的调控机制奠定基础。

Objective To analyze the expression of miRNA-204 in hippocampus of epileptic mice based on gene chip.Methods Differential genes were detected by microarray in hippocampal tissues of epileptic mice,and their target genes were enriched and pathway analyzed by bioinformatics.Results The expression of miRNA-204,a differential gene associated with epilepsy,was down-regulated,and 457 related target genes were predicted.The function of 27 genes(P<0.01)and 16 signaling pathways(P<0.05)were obtained by enrichment analysis.Conclusion Through analysis,the effect of miRNA-204 down-regulation on epileptic mice was clarified,and the miRNA-204 related pathway Wnt was analyzed,laying a foundation for further study on the regulation mechanism of miRNA-204 on epilepsy.