血清微小RNA-181b的表达与子痫前期患者白介素-6、白介素-10、白介素-17的相关性

Correlation between serum microRNA-181 b expression and interleukin-6,interleukin-10,interleukin-17 in patients with preeclampsia

目的观察子痫前期(PE)患者血清微小RNA(miR)-181b的表达,并分析其与白介素(IL)-6、IL-10、IL-17的关系。方法选取2018年10月至2020年12月海南医学院第二附属医院收治的96例PE患者作为研究对象,并根据病情程度分为非重度PE(N-PE)组和重度PE(S-PE)组,各48例。询问并记录患者的基线资料;检测患者入院时血清miR-181b及血清IL-6、IL-10、IL-17等炎性因子水平;分析血清miR-181b的表达与PE患者IL-6、IL-10、IL-17的关系,并进一步探究血清miR-181b与PE病情的关系及其对评估S-PE发生风险的价值。结果S-PE组患者入院时血清miR-181b、IL-6、IL-17水平均高于N-PE组,血清IL-10水平低于N-PE组,差异具有统计学意义(P<0.05);相关性检验发现,血清miR-181b的表达与PE患者IL-6、IL-17水平呈正相关(r>0,P<0.05),与血清IL-10水平呈负相关(r<0,P<0.05);回归分析发现,入院时血清miR-181b的过表达与PE患者病情存在一定关系,其可作为S-PE发生的风险因子(P<0.05);绘制受试者工作特征(ROC)曲线,入院时血清miR-181b的表达评估S-PE发生风险的曲线下面积(AUC)为0.896,有一定的评估价值。结论血清miR-181b的表达与PE患者IL-6、IL-17水平呈正相关,与血清IL-10水平呈负相关,且其过表达与PE患者病情存在一定关系,可作为S-PE发生的风险因子。

Objective To observe the expression of serum microRNA(miR)-181 b in patients with preeclampsia(PE), and to analyze its relationship with interleukin(IL)-6, IL-10 and IL-17. Methods 96 patients with PE admitted to the Second Affiliated Hospital of Hainan Medical University from October 2018 to December 2020 were selected as the research subjects. According to the severity of the disease, the patients were divided into non-severe PE(N-PE) group and severe PE(S-PE) group, with 48 cases in each group.Asked and recorded the baseline data of patients, the serum miR-181 b level of patients at admission was detected;the levels of serum IL-6, IL-10, IL-17 inflammatory factors were detected at admission;the relationship between serum miR-181 b expression and IL-6, IL-10, IL-17 in patients with PE was analyzed, the relationship between serum miR-181 b and disease condition of preeclampsia and its value in evaluating the risk of S-PE were further explored. Results The levels of serum miR-181 b, IL-6 and IL-17 of patients in S-PE group at admission were higher than those in N-PE group, and the level of serum IL-10 was lower than that in N-PE group, the differences were statistically significant(P<0.05);correlation test showed that the expression of serum miR-181 b was positively correlated with the levels of IL-6 and IL-17 in PE patients(r>0, P<0.05), and negatively correlated with the level of serum IL-10(r<0, P<0.05);regression analysis showed that the overexpression of miR-181 b at admission was related to the disease condition of PE patients, which might be a risk factor for the occurrence of S-PE(P<0.05);the receiver operating characteristic(ROC) curve was drawn, the area under the curve(AUC) of serum miR-181 b overexpression at admission in evaluating the risk of S-PE was 0.896, which had certain evaluation value. Conclusions The expression of serum miR-181 b is positively correlated with the levels of IL-6 and IL-17 in PE patients, and negatively correlated with the level of serum IL-10. Its overexpression at admission is related to the disease condition of PE patients, which may be a risk factor for the occurrence of S-PE.