卵巢子宫内膜异位囊肿组织B淋巴细胞瘤-2相关X、B淋巴细胞瘤-2蛋白的表达及其与超声引导下无水乙醇介入治疗术后复发的关系分析

Expressions of Bax and Bcl-2 proteins in ovarian endometriosis cysts and its relationship with recurrence after ultrasound-guided anhydrous ethanol intervention

目的检测卵巢子宫内膜异位囊肿组织B淋巴细胞瘤-2相关X(Bax)、B淋巴细胞瘤-2(Bcl-2)蛋白的表达,并分析其与超声引导下无水乙醇介入治疗术后复发的关系。方法回顾性选取2016年1月至2017年6月于徐州市中心医院就诊的215例卵巢子宫内膜异位囊肿患者的临床资料纳入研究组,实施超声引导下无水乙醇介入治疗并于术中留取患者的卵巢子宫内膜异位囊肿组织;另选取同期同一医院就诊的206例子宫肌瘤患者的临床资料纳入对照组,均于行手术治疗时经活检穿刺术留取患者的子宫内膜组织。采用免疫组化法检测两组患者组织中Bax、Bcl-2蛋白的表达并进行比较;分析卵巢子宫内膜异位囊肿组织中Bax、Bcl-2蛋白表达与术后复发的关系。结果研究组增生期、分泌期Bax蛋白阳性表达率及总阳性表达率均低于对照组,Bcl-2蛋白阳性表达率及总阳性表达率均高于对照组,差异具有统计学意义(P<0.05);研究组术后复发率为22.33%,复发患者囊壁厚度≥0.3cm、入院时血清糖类抗原125(CA125)阳性、术中无水乙醇留置时间<15min、Bax蛋白阴性表达、Bcl-2蛋白阳性表达占比均高于未复发患者,差异具有统计学意义(P<0.05),且均为研究组复发的独立危险因素(P<0.05)。结论卵巢子宫内膜异位囊肿组织中Bax蛋白阳性表达率低,Bcl-2蛋白阳性表达率高,二者与患者行超声引导下无水乙醇介入治疗术后复发有关。

Objective To detect the expression of B-lymphoma-2-associated X(Bax)and B-lymphoma-2(Bcl-2)proteins in ovarian endometrioma cyst tissue and analyze its relationship with the recurrence after ultrasound-guided anhydrous ethanol interventional therapy.Methods The clinical data of 215 patients with ovarian endometriosis cyst who were admitted to Xuzhou Central Hospital from January 2016 to.June 2017 were retrospectively selected as the subjects of the study group.All patients were treated with ultrasound-guided anhydrous ethanol intervention and the ovarian endometriosis cyst tissues were collected during the operation.In addition,the clinical data of 206 patients with uterine fibroids treated in the same hospital during the same period were included in the control group.Endometrial tissues of patients were collected by biopsy and puncture during surgical treatment.Immunohistochemistry was used to detect the expression of Bax and Bcl-2 proteins in the tissues of the two groups and compare them and to analyze the relationship between the expression of Bax and Bcl-2 proteins in ovarian endometriosis cyst tissue and postoperative recurrence.Results The positive expression rate and total positive expression rate of Bax protein in the proliferation and secretion stages of the study group were lower than those in the control group,and the positive expression rate and total positive expression rate of Bcl-2 protein were higher than those in the control group,with statistical significance(P<0.05).In the study group,the postoperative recurrence rate was 22.33%,the thickness of capsule wall≥0.3cm,the positive serum carbohydrate antigen 125(CA125)at admission,the intraoperative indent time of anhydrous ethanol<15min,the negative expression rate of Bax protein and the positive expression rate of Bcl-2 protein were higher than those in the non-recurrence patients,and the differences were statistically significant(P<0.05).All were independent risk factors for recurrence in the study group(P<0.05).Conclusions The positive expression rate of Bax protein in ovarian endometrioma cyst tissue is low,and the positive expression rate of Bcl-2 protein is high,both of which are related to the recurrence of patients after ultrasound-guided anhydrous ethanol interventional therapy.