大球盖菇新品种“中菌金球盖1号”的基因组Survey分析

Genome Survey Analysis of New Variety ZJJQG001 of Stropharia rugosoannulata

大球盖菇(Stropharia rugosoannulata)是一种营养丰富、口味鲜美的食用菌,也是极具开发潜力的药用真菌。为了深入对金黄色大球盖菇的子实体发育、颜色调控等遗传机理开展研究,为金黄色大球盖菇基因组的精细图谱、分子育种等研究打下基础,采用第二代高通量测序技术对金黄色大球盖菇品种“中菌金球盖1号”开展全基因组Survey分析。结果表明,金黄色大球盖菇品种“中菌金球盖1号”的基因组大小约为55.20 Mb,测序深度为140×,杂合率为0.80%,(G+C)含量为47.25%;基因组初步组装后,得到25802条重叠群(Contigs),Contig N50为14.923 Kb。该研究结果对于开展金黄色大球盖菇的颜色调控等遗传机理研究具有重要意义,同时为金黄色大球盖菇下一步的基因组精细图谱的构建和分子育种提供依据。

Stropharia rugosoannulata is an edible fungus with rich nutrition,delicious taste and is also a medicinal fungus with great potential for development.The genome of S.rugosoannulata is the base to study the molecular colorful regulation mechanism and molecular breeding.In the study,the genome of S.rugosoannulata was measured by next-generation sequencing.The results showed that,the genome size of S.rugosoannulata was 55.20 Mb and the sequencing depth was 140×,the heterozygosity rate was 0.80%,(G+C)content was 47.25%.25802 scaffolds were obtained which was assembled and the Scaffold N50 was 14.923 Kb.According to the evaluation of the various indicators of S.rugosoannulata genome,it will provide a basis for colorful regulation mechanism and provide valuable data for molecular breeding.