基于代谢组学辨析广西长寿饮食模式对机体的有益影响

Exploring the Beneficial Effects of Guangxi Longevity Dietary Patterns on Human Body Based on Metabolomics

为了深入探究饮食与机体健康状态的关系,本研究试图从代谢组学的角度,评估所构建的广西长寿饮食模式对人体的有益影响。筛选志愿者在经过2周习惯性饮食后,高依从性地遵循这种饮食模式2周,在习惯性饮食前,广西长寿饮食前(习惯性饮食后)和广西长寿饮食后3次分别采集志愿者的血液和粪便样品,进行代谢组学分析。归纳所得结果发现,广西长寿饮食干预后志愿者血清组氨酸、甘油、α-葡萄糖和β-葡萄糖相对丰度显著增加(P<0.05),胆碱和乳酸的相对丰度显著降低(P<0.05)。此外还发现,粪便中的乙酸盐和丁酸盐含量显著上升(P<0.05)。代谢物组富集分析和途径分析结果表明,这些代谢物的变化可能与糖酵解/糖异生途径、丙酮酸代谢和半乳糖代谢3条途径密切相关。通过对差异代谢物的分析,认为广西长寿饮食模式对改善机体炎症水平、调节机体健康状态、预防机体罹患心血管疾病具有一定的作用。上述结果也从代谢组学角度对饮食促进机体健康长寿的关系提供了一定机制方面的阐释。

To explore the relationship between diet and human health,this study aimed to evaluate the beneficial effects of the existing Guangxi longevity dietary pattern on the human body from metabolomics.After 2 weeks of habitual diet,the selected volunteers followed this dietary pattern with high adherence for the same period.The blood and fecal samples were collected 3 times then performed metabolomics analysis,including before the habitual diet,the beginning(which was also the ending point of habitual diet)and the end of Guangxi longevity dietary pattern.The results showed that after the Guangxi longevity dietary intervention of volunteers,the relative abundance of serum histidine,glycerol,α-glucose andβ-glucose was significantly increased(P<0.05),but the relative richness of choline and lactic acid reduced obviously(P<0.05).In addition,the fecal acetate and butyrate increased apparently(P<0.05).Moreover,the analysis of metabolome enrichment and pathway revealed that these metabolites’variations might be closely related to three pathways,including glycolysis/gluconeogenesis pathway,pyruvate metabolism,and galactose metabolism.Through the analysis of differential metabolites,it was considered that Guangxi longevity dietary pattern played a promoted role in improving the level of inflammation,regulating the health status and preventing the organism from developing cardiovascular diseases.The above results also provide a mechanical explanation of the relationship between dietary patterns and healthy longevity from human metabolism.