基于体外消化模型研究板栗壳和囊衣原花青素类物质消化前后的组成差异

Composition Differences of Procyanidins in Chestnut Shell and Pellicle before and after Digestion Based on in Vitro Digestion Model

本文以板栗壳和囊衣为原料,采用基于超高效液相色谱-串联质谱(UPLC-MS/MS)技术的广泛靶向代谢组学,分析样品中原花青素物质经过模拟消化后的组成变化。结果显示,板栗壳和囊衣及其代谢物中共鉴定出33种原花青素类代谢物,差异代谢物分析显示,消化后板栗壳中表儿茶素、原花青素C2及原花青素A1等16种原花青素类化合物均呈显著(P<0.05)下调;板栗囊衣中没食子儿茶素-儿茶素-儿茶素、没食子儿茶素及没食子酸儿茶素没食子酸酯等17种原花青素类化合物均呈显著(P<0.05)下调。其中,下调最显著(P<0.05)的是没食子儿茶素-儿茶素-儿茶素,在体外胃肠消化后分别降低了98.23%(板栗壳)和97.86%(囊衣)。综上,板栗壳和囊衣中原花青素类物质在消化过程中稳定性较差,未来的研究中可采用设计靶向释放载体对其进行包埋等方式,以提高其生物利用率。

In this study,the composition changes of procyanidins in chestnut shell and pellicle after simulated digestion were analyzed by extensive targeted metabolomics based on ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The results showed that a total of 33 procyanidin metabolites were identified in chestnut shell,pellicle and their metabolites.Differential metabolite analysis showed that 16 procyanidin compounds,including epicatechin,procyanidin C2 and procyanidin A1 were significantly(P<0.05)down-regulated in chestnut shell after digestion.Seventeen procyanidins,including gallocatechin-catechin-catechin,gallocatechin and gallocatechin gallate,were significantly(P<0.05)down-regulated in chestnut pellicle after digestion.Among them,the most significant(P<0.05)down-regulation was gallocatechin-catechin-catechin,which was reduced by 98.23%(chestnut shell)and 97.86%(chestnut pellicle)after gastrointestinal digestion in vitro.In conclusion,the stability of proanthocyanidins in chestnut shell and pellicle was poor during digestion.In future studies,targeted release carriers could be designed to encapsulate them,so as to improve their bioavailability.