超声辅助低共熔溶剂提取新疆红枣中环磷酸腺苷的工艺优化

Optimization of Ultrasound-Assisted Extraction of Cyclic Adenosine 3',5'-Monophosphate from Xinjiang Jujube by Deep Eutectic Solvent

针对新疆红枣,采用绿色、高效的低共熔溶剂(Deep Eutectic Solvent,DES)为提取剂,通过超声波辅助技术提取其中的功能性成分--环磷酸腺苷(cyclic adenosine 3',5'-monophosphate,cAMP)。研究低共熔溶剂的摩尔比、含水量以及料液比、超声时间、超声温度与cAMP提取量的关系,通过单因素实验和响应面优化试验,得出新疆红枣中cAMP提取的最佳条件为:氯化胆碱与丙三醇摩尔比为1:3,DES体系含水量为44%,红枣粉末与DES的料液比为1:35 g/mL,超声时间为45 min,超声温度为45℃,此时与同等超声条件下的水提法和醇提法相比,低共熔溶剂法提取cAMP的含量最高为(284.15±0.06)μg/g。因此,选用超声波辅助低共熔溶剂提取新疆红枣中的cAMP是获得较高提取量的一种新型、高效和安全的方法。

Using green and highly efficient deep eutectic solvent(DES)as the extraction agent,this study extracted the functional ingredient—cyclic adenosine 3',5'-monophosphate(cAMP)from Xinjiang jujube through ultrasound-assisted technology.The relationships between the molar ratio of deep eutectic solvent,water content of deep eutectic solvent,solid-liquid ratio,ultrasonic time,ultrasonic temperature and extraction amount of cAMP were studied.Through single factor experimentas and response surface optimization experiment,the optimal conditions for cAMP extraction from Xinjiang jujube were as follows:The molar ratio of choline chloride to glycerol was 1:3,the water content of DES system was 44%,the solid-liquid ratio of jujube powder to DES was 1:35 g/mL,the ultrasonic time was 45 min,and the ultrasonic temperature was 45℃.At this time,compared with water extraction and alcohol extraction under the same ultrasonic conditions,the highest content of cAMP extracted by deep eutectic solvent was(284.15±0.06)μg/g.Therefore,ultrasonic-assisted deep eutectic solvent extraction of cAMP in Xinjiang jujube was a new,efficient and safe method to obtain a higher extraction amount.