绿鳍马面鲀微卫星标记开发及在丝背细鳞鲀中的通用性检测

Development of microsatellite markers for Thamnaconus modestus and their transferability in Stephanolepis cirrhifer

为充分挖掘绿鳍马面鲀(Thamnaconus modestus)基因组资源,开发可用于群体遗传学研究的分子标记,本研究利用NCBI数据库中公布的绿鳍马面鲀全基因组序列筛查微卫星位点并在野生群体中验证,在85个选取的位点中筛选得到30个新的多态性较高的微卫星标记。每个微卫星标记的等位基因数为4~16,平均为8.5个;观测杂合度范围为0.207~0.916,平均值为0.685;期望杂合度范围为0.315~0.971,平均值为0.756;多态信息含量范围为0.301~0.898,平均值为0.716,其中属于高度多态的位点有26个,占总位点数的86.67%;经过Bonferroni校正后,有7个位点显著偏离Hardy-Weinberg平衡。将30个绿鳍马面鲀多态性微卫星标记在丝背细鳞鲀(Stephanolepis cirrhifer)中进行通用性检测,其中13个位点成功扩增,9个位点具有多态性。本研究开发的微卫星标记可用于绿鳍马面鲀及相关物种的遗传多样性分析、QTL定位以及系统进化等研究。

In this study,microsatellite loci in the complete genome sequence of T.modestus(published in NCBI)were screened and verified in a wild population.The aim is to develop molecular markers for population genetics research and fully exploit the genomic resources of Thamnaconus modestus.Thirty highly polymorphic,novel mi-crosatellite markers were identified in 85 selected loci.The number of alleles ranged from 4 to 16,with an average of 8.5.The observed heterozygosity ranged from 0.207 to 0.916,with an average of 0.685.The expected heterozy-gosity ranged from 0.315 to 0.971,with an average of 0.756.The polymorphic information content ranged from 0.301 to 0.898,with an average of 0.716.Of the 30 microsatellite loci identified,26(86.67%)were highly poly-morphic,and 7 significantly deviated from the Hardy–Weinberg equilibrium after Bonferroni correction.The trans-ferability of these polymorphic microsatellite markers was assessed in the closely related species Stephanolepis cirrhifer,in which 13 loci were successfully amplified,and 9 were found to be polymorphic.The microsatellite markers developed in this study can be used in genetic diversity analysis,QTL mapping,and phylogenic studies of T.modestus and related species.