窄带紫外LED照射对大鼠骨代谢的影响

Beneficial Effects of Short-Wavelength Narrow Band Ultraviolet Irradiation Delivered Using an LED Device on Bone Metabolism in Rats

背景:紫外线是维持骨骼健康的关键条件之一,但由于传统的紫外线光谱较宽,在骨质疏松症领域并未引起足够的重视。材料与方法:在研究中,建立了一种新设计的窄带光谱LED器件,以研究紫外LED对骨质疏松大鼠骨代谢、骨形态和皮肤的影响。共设置了去卵巢大鼠(n=24)和去卵巢大鼠(n=36)。然后将去卵巢大鼠分为假手术组(Sham,n=12)和检测模型组(Sham,n=12)。将去卵巢大鼠分为LED照射组(LED,n=12)、未处理组(OVX,n=12)或检测模型组(OVX,n=12)。LED辐照参数(0.8 mW·cm^(-2),1000 s,每周两次)。结果:与假手术组相比,维生素D、mRNA、血清ALP、血清25(OH)D、血清P1NP的表达明显升高,血清PTH和血清TRAP的表达明显降低。大鼠皮肤照射前后p53基因未见明显变化。结论:研究结果表明,新型紫外LED器件照射可显着提高血液中25(OH)D水平,促进骨形成,抑制骨吸收,且对大鼠皮肤无不良影响。

Background:Ultraviolet radiation is one of the key conditions to maintaining bone health,but it has not been paid enough attention to in the field of osteoporosis because of the wide traditional ultraviolet spectrum.With the development of LED technology,all kinds of narrowband LED spectra can be adjusted arbitrarily.Materials and methods:In this study,we established a newly designed LED device with a narrow band spectrum to investigate the effects of ultraviolet LED on bone metabolism,bone morphology and skin of osteoporotic rats.We have set up a total of unovariectomized rats(n=24)and ovariectomized rats(n=36).The unovariectomized rats were then divided into the sham operation group(Sham,n=12)and the detection model group(Sham,n=12).The ovariectomized rats were divided into either the LED irradiation group(LED,n=12),the no treatment group(OVX,n=12),or the detection model group(OVX,n=12).The LED Irradiation parameters(0.8 mW·cm^(-2)-2,1000s,twice a week).Results:Compared with the sham group,the expression of vitamin D mRNA,serum bone ALP,serum 25(OH)D3,and serum P1NP increased significantly,the serum PTH and serum TRAP decreased significantly.Conclusion:Our results show that irradiation with the new ultraviolet LED device can significantly increase the level of 25(OH)D3in blood,promote bone formation and inhibit bone resorption without adverse effects on rat skin.