lncRNA MEG3基因多态性与不同宫颈病变的相关性研究

The association between polymorphisms in long non-coding RNA MEG3 and the different cervical lesions

目的:探讨MEG3 rs10132552和rs7158663单核苷酸多态性(single nucleotide polymorphism,SNP)与不同宫颈病变患者发病风险的关系。方法:2018年11月到2020年11月,收集328例宫颈病变患者的外周静脉血及相关临床信息为病例,包括210例宫颈上皮内病变(cervical intraepithelial neoplasia,CIN)患者和118例宫颈癌(cervical cancer,CC)患者,以同期住院的345例无宫颈病变患者作为对照组,采用TaqMan RT-PCR方法进行基因分型,采用χ^(2)检验和logistic回归分析SNP位点与不同级别宫颈病变的相关性。结果:与对照组相比,MEG3 rs10132552 CT+CC基因型在宫颈病变组中分布频率显著降低(P<0.05),其发病风险为0.701(95%CI:0.515~0.954);且在>50岁患者中,携带MEG3 rs10132552 CT+CC基因型发生宫颈病变的风险显著降低(OR=0.634,95%CI:0.412~0.978,P<0.05)。将宫颈病变组分为宫颈上皮内病变组和宫颈癌组进行分层分析发现,该位点CT+CC基因型主要降低了宫颈癌组的发病风险(OR=0.520,95%CI:0.333~0.811,P<0.05);进一步进行临床进展相关性分析,未发现MEG3 rs10132552与不同级别宫颈上皮内病变、不同病理类型、临床分期的宫颈癌具有相关性(P>0.05)。未发现MEG3 rs7158663位点与不同级别宫颈病变具有相关性(P>0.05)。结论:MEG3 rs10132552 SNP与宫颈癌的发病风险相关,其CT+CC基因型可能是宫颈癌发生的保护因素。

Objective:To investigate the association between single nucleotide polymorphisms(SNP)of MEG3 rs10132552 and rs7158663 and the risk of cervical lesions of different grades.Methods:From November 2018 to November 2020,the peripheral venous blood samples and clinical information were collected from 328 patients with cervical lesions treated at the hospital,including 210 patients with cervical intraepithelial neoplasia(CIN)and 118 patients with cervical cancer(CC).345 patients without cervical lesions hospitalized in the same period were healthy controls.TaqMan RT-PCR was used for genotyping,and the Chi-square test and logistic regression were used to analyze the association of single nucleotide the polymorphism with cervical lesions of different grades.Results:Compared with the control group,the distribution frequency of CT+CC genotype in MEG3 rs10132552 in the case group was significantly lower(P<0.05),and its risk was 0.701(95%CI:0.515~0.954).In patients over 50 years of age,the risk of cervical lesions with MEG3 rs10132552 CT+CC genotype was significantly reduced(OR=0.634,95%CI:0.412~0.978,P<0.05).The case group was divided into CIN group and CC group.Stratified analysis showed that the CT+CC genotype at this locus mainly reduced the risk of the CC group(OR=0.520,95%CI:0.333~0.811,P<0.05).Further clinical progression correlation analysis showed that MEG3 rs10132552 was not correlated with different grades of cervical intraepithelial neoplasia,different pathological types,and clinical stages of cervical cancer(P>0.05).There was no correlation between MEG3 rs7158663 and cervical lesions of different grades(P>0.05).Conclusion:MEG3 rs10132552 SNP is associated with the risk of cervical cancer,and the CT+CC genotype may be a protective factor for cervical cancer.