新型绿色溶剂1-庚基-3-甲基咪唑氯盐离子液体对小鼠急性暴露毒性研究

Toxicological evaluation for acute exposure of a new green solvent,1-heptyl-3-methylimidazolium chloride ionic liquid to mice

目的 探究烷基咪唑离子液体1-庚基-3-甲基咪唑氯盐(1-heptyl-3-methylimidazolium chloride,C[MIM]Cl)对小鼠急性暴露的毒性作用。方法 采用上下法,研究了C[MIM]Cl对小鼠的急性经口毒性并对死亡小鼠进行解剖病理学观察;采用最大限量法确定小鼠急性经皮毒性的范围,并评价其毒性等级;采用急性眼刺激法,考察C[MIM]Cl对小鼠眼和黏膜原发性刺激和腐蚀作用。结果 研究表明C[MIM]Cl经口暴露对小鼠的肝脏、肾脏表现出明显毒性损伤作用,其半数致死量(50%lethal dose, LD_(50))为119.00 mg/(kg·bw), 95%可信区间为87.95~202.00 mg/(kg·bw),属于中等毒性。同时急性经皮试验表明其LD_(50)在200~1000 mg/(kg·bw)之间。急性眼刺激试验表明, C_(7)[MIM]Cl对小鼠眼部有中度刺激性,甚至可以导致小鼠死亡。结论 C_(7)[MIM]Cl属于中等毒性化合物,在食品领域应用的安全性亟待开展更广泛的毒理学评价。

Objective To investigate the acute exposure toxicity of alkyl imidazole ionic liquid 1-heptyl-3-methylimidazolium chloride(C[MIM]Cl) in mice.Method Acute oral toxicity of C[MIM]Cl to mice was studied by up-down method and the dead mice were executed anatomicaland pathological observation;the maximum limit method was used to determine thetoxicityrange and category for acute dermal toxicity;acute eye stimulation method was used to investigate the primary irritation and corrosion of mouse eyes and mucous membrane induced by C[MIM]Cl.Results The study showed that oral exposure of C[MIM]Cl exhibited significant toxic damage to the liver and kidney of mice,with the estimated 50% lethal dose(LD_(50)) of 119.00 mg/(kg·bw) and the 95% confidence interval of 87.95-202.00 mg/(kg·bw),rated as moderate toxicity.Meanwhile,acute percutaneous tests showed that LDof dermal toxicity was between 200 and 1000 mg/(kg·bw).Acute eye stimulation test showed that C_(7)[MIM]Cl had moderate ocular irritation,and could even lead to the death of mice.Conclusion C_(7)[MIM]Cl is a moderate hazardous chemical and warrant to be executed a wider toxicological evaluation as for its safety application in food industry.