电化学分析法检测动物源食品中氯丙嗪的3种前处理方法比较

Comparison of 3 kinds of pretreatment methods for determination of chlorpromazine in animal-derived foods by electrochemical analysis

目的 比较电化学分析法检测动物源食品中氯丙嗪的3种不同前处理方法。方法 样品分别采用溶剂提取法、QuEChERS法和固相萃取法(solid-phase extraction, SPE)进行了电化学检测比较分析。结果 猪肉、鸡肉、牛肉、猪肝、虾肉和蜂蜜可用溶剂提取法进行处理,氯丙嗪的加标回收率均在81.6%以上;而鱼肉和鸡蛋样品需采用QuEChERS法进行处理,回收率分别达到88.5%~95.8%和89.7%~98.2%;牛奶样品用溶剂提取法和QuEChERS法处理时均有基质干扰,采用固相萃取法进行净化后,回收率达到80.6%~93.0%。结论 不同类型的动物源食品需分别采用适宜的前处理方法才能获得满意的测定结果。本研究可为不同类型动物源食品中氯丙嗪电化学检测方法的应用提供参考。

Objective To compare the 3 kinds of different pretreatment methods for the determination of chlorpromazine in animal-derived foods by electrochemical analysis.Methods The samples were subjected to electrochemical detection and comparative analysis by solvent extraction method,QuEChERS method and solid-phase extraction(SPE).Results Pork,chicken,beef,liver,shrimp and honey could be processed by solvent extraction method,and the recovery rates of chlorpromazine were all above 81.6%;while fish and egg samples were processed by QuEChERS method,and the recoveries were 88.5%-95.8% and 89.7%-98.2%,respectively;the milk samples had matrix interference when treated by solvent extraction method and QuEChERS method.After purification by SPE method,the recoveries reached 80.6%-93.0%.Conclusion Different types of animal-derived foods need appropriate pretreatment methods to obtain satisfactory determination results.This study can provide reference for the application of electrochemical detection methods of chlorpromazine in different types of animal-derived foods.