FTO基因rs9939609多态性与青少年肥胖及血细胞分析参数的相关性

Study on correlation between rs9939609 polymorphism of FTO gene and obesity and hematological indexes in adolescents

目的 探讨脂肪量与肥胖相关(fat mass and obesity-associated,FTO)基因多态性与青少年人群肥胖及血细胞分析参数之间的关系。方法 选取2020年1月至12月于湖北中医药大学黄家湖医院体检的青少年人群541例作为研究对象,并进一步分为体重过轻组(BMI<18.5)、正常组(BMI 18.5~23.9)、超重组(BMI 24.0~27.9)和肥胖组(BMI≥28)。应用Sanger测序法测定各组FTO基因rs9939609位点的基因型,并分析FTO基因多态性对肥胖及各血细胞分析参数的影响。结果 血细胞分析参数中白细胞(WBC)、单核细胞(MONO)、中性粒细胞(NEUT)、淋巴细胞(LYMPH)、嗜酸性粒细胞(EO)、红细胞(RBC)、红细胞压积(HCT)、血红蛋白(Hb)、红细胞分布宽度(RDW-SD)、平均血红蛋白浓度(MCHC)、红细胞平均体积(MCV)、血小板(PLT)和血小板压积(PCT)数值在不同BMI分组间的差异均具有统计学意义(F分别为10.931、9.946、4.412、8.980、4.584、24.646、19.747、20.498、5.122、6.914、3.427、4.215、3.094,P均<0.05)。3种基因型频率在肥胖组与正常组之间比较差异有统计学意义(χ^(2)=19.845,P<0.01)。AA基因型个体的WBC、EO和PLT数值明显高于TA和TT基因型个体(F分别为4.088、5.087、3.047,P均<0.05),且FTO基因rs9939609位点AA基因型是影响EO数值的独立指标[OR=0.092 (0.020~0.165),t=2.507,P=0.013]。AA基因型个体发生肥胖的风险是TT基因型携带者的8.261倍(χ^(2)=19.34,P=0.000),A等位基因的肥胖风险是T等位基因的1.721倍(χ^(2)=7.603,P=0.006)。结论 FTO基因rs9939609位点多态性与肥胖及血细胞分析参数之间存在相关性。

Objective To investigate the relationship between fat mass andobesity-associated(FTO)gene polymorphism and obesity and hematological parameters in adolescents.Methods A total of 541 adolescents who underwent physical examination in Huangjiahu Hospital during 2020 was selected as the research subjects who were divided into underweight group(BMI<18.5),normal group(BMI 18.5-23.9),overweight group(BMI 24.0-27.9)and obese group(BMI≥28).Sanger sequencing method was used to determine the genotype of rs9939609 of FTO gene,and the effects of FTO gene polymorphism on obesity and hematological parameters were analyzed.Results The hematological parameters including the count of white blood cell(WBC),monocyte(MONO),neutrophils(NEUT),lymphocyte(LYMPH),eosinophils(EO),red blood cell(RBC)and platelet(PLT)hematocrit(HCT),hemoglobin(Hb),red cell distribution width-standard deviation(RDW-SD),the values of mean corpuscular hemoglobin concentration(MCHC),mean corpuscular volume(MCV)and platelet volume(PCT)were significantly different among different BMI groups(F=10.931,9.946,4.412,8.980,4.584,24.646,19.747,20.498,5.122,6.914,3.427,4.215,3.094,P<0.05).There were significant differences for the frequency of the three genotypes between the obese and the normal group(χ^(2)=19.845,P<0.01).The counts of WBC,EO and PLT in AA genotypes were significantly higher than those of TA and TT genotypes(F=4.088,5.087 and 3.047,P<0.05).The rs9939609 AA genotype in FTO was an independent index affecting EO count,OR[0.092(0.020-0.165)],t=2.507,P=0.013.The risk of obesity in AA genotype was 8.261 times higher than that in TT genotype carriers(χ^(2)=19.34,P=0.000),and the risk of obesity in A allele was 1.721 times higher than that in T allele(χ^(2)=7.603,P=0.006).Conclusion A correlation between rs9939609 polymorphism of FTO gene and obesity as well as hematological parameters should be present.