白豆蔻对阿霉素肾病所致大鼠肾损伤和肾纤维化的保护作用及机制

Protective effect and mechanism of amomun kravanh on renal injury and fibrosis induced by adriamycin nephropathy in rats

目的 探究白豆蔻对阿霉素肾病所致大鼠肾损伤和肾纤维化的保护作用及可能机制。方法30只SD大鼠随机分成对照组(Control组)、阿霉素肾病组(AN组)和白豆蔻组(AK组),每组10只。HE染色观察肾组织形态学变化;Masson染色观察肾组织纤维化;ELISA方法检测大鼠血清血肌酐和尿素氮水平及肾组织匀浆MDA和SOD水平;Western blot检测肾组织凋亡相关蛋白和Sirt1/PGC-1α信号通路相关蛋白表达。结果 白豆蔻能改善大鼠肾组织形态,降低组织纤维化,降低血清血肌酐和尿素氮水平,抑制肾组织氧化应激反应,降低肾组织细胞凋亡,激活Sirt1/PGC-1α信号通路。结论 白豆蔻改善阿霉素肾病所致大鼠肾损伤和肾纤维化,其作用机制可能与调控Sirt1/PGC-1α信号通路有关。

Objective To investigate the protective effect of amomun kravanh on renal injury and fibrosis induced by adriamycin nephropathy in rats and its possible mechanism. Methods Thirty SD rats were randomly divided into control group, adriamycin nephropathy group(AN group) and amomun kravanh group(AK group), with 10 rats in each group. HE staining was used to observe the morphological changes of renal tissues. Masson staining was used to observe renal fibrosis. The levels of serum creatinine and urea nitrogen and the levels of MDA and SOD in renal tissue homogenate were detected by ELISA. The expressions of apoptosis-related proteins and Sirt1/PGC-1α signaling pathway related proteins in renal tissues were detected by Western blot. Results Amomun kravanh can improve renal morphology, decrease tissue fibrosis, decrease serum creatinine and urea nitrogen levels, inhibit renal oxidative stress response, decrease renal cell apoptosis, and activate Sirt1/PCC-1 α signaling pathway. Conclusion Amomun kravanh improved renal injury and fibrosis in rats induced by adriamycin nephropathy, which may be related to SIRT1/PGC-1α signaling pathway.