20℃和25℃条件下秀丽隐杆线虫衰老过程中磷酸化蛋白质组学的研究

Phosphoproteomic Study in C.elegans during Aging Process under 20℃and 25℃

衰老过程受到包括温度在内的众多环境因素的影响。蛋白质磷酸化是重要的翻译后修饰,调控多种生命活动,在衰老过程中也起着重要作用。秀丽隐杆线虫(Caenorhabditis elegans,C.elegans)是经典的衰老研究的模式生物。为了探究蛋白质磷酸化修饰在不同温度下衰老过程中的作用,我们对20℃(常温)和25℃(高温)培养条件下成年时期第1天、第5天、第10天的秀丽隐杆线虫样品进行了磷酸化组学分析工作。我们采用基于DIA的非标定量分析方法,系统地比较了不同样品间的磷酸化组学差异。共9145条高可信度的磷酸化肽段被鉴定到,对应3317个磷酸化蛋白质。经过筛选,最终6624条磷酸化肽段被定量到,其中有1093条显著变化的磷酸化肽段,来自于858个磷酸化蛋白质,包含1426个磷酸化位点,通过进一步的生物信息学分析,揭示了衰老过程中的磷酸化变化规律。

Aging process is affected by many environmental factors including temperature.As an important post-translational modification,protein phosphorylation regulates many biological processes including aging.C.elegans is one of the classic model organisms in aging research.To investigate the role of protein phosphorylation during aging process under different temperatures,C.elegans adult samples from Day 1(fertile young adults),Day 5(early aging)and Day 10(aging)cultured at 20℃(normal temperature)and 25℃(higher temperature)were anaylsed by phosphoproteomic approach,DIA-based label-free quantification method was used to systematically investigate the phosphoproteomic changes.In total 9145 phosphopeptides from 3317 phosphoproteins were identified with high confidence,and 6624 phosphopeptides were quantified from 2997 phosphoproteins after data filtering.1093 significantly regulated phosphopeptides with 1426 phosphosites from 858 phosphoproteins were found,and phosphoproteomic changes during aging process in C.elegans were revealed through bioinformatics analysis.