花期耧斗菜乙醇粗提液对桃蚜的室内触杀活性及田间药效

Evaluation of the ethanol crude extract of Aquilegia viridiflora Pall.at blooming stage against Myzus persicae(Sulzer)for laboratory contact toxicity and field efficacy

【目的】探究花期耧斗菜不同器官乙醇粗提液对桃蚜的触杀活性。【方法】采用浸虫法测定花期耧斗菜叶部、茎部、根部乙醇粗提液对桃蚜的室内触杀活性,并选择花期耧斗菜全株乙醇粗提液开展田间药效试验。【结果】花期耧斗菜叶部、茎部、根部乙醇粗提液浓度为50.0 mg/mL、作用72 h时,对桃蚜的致死率和校正死亡率均达到70.00%以上,且处理间无显著差异(P>0.05)。毒力回归方程叶部为y=2.48+1.78x(R^(2)=0.99)、茎部为y=4.84+0.47x(R^(2)=1.00)、根部为y=3.03+1.91x(R^(2)=0.91)。花期耧斗菜全株乙醇粗提液25.0 mg/mL在田间施用后,7 d桃蚜防治效果达70.31%。【结论】花期耧斗菜叶部、茎部、根部乙醇粗提液对桃蚜的触杀效果相当,其杀虫有效成分较为均匀地分布于植物全株;花期耧斗菜全株乙醇粗提液对桃蚜具有较好的田间防治效果。

【Objective】To evaluate the contact toxicity of ethanol crude extracts from various Aquilegia viridiflora Pall.organs at blooming stage to Myzus persicae(Sulzer).【Methods】The laboratory contact toxicity of ethanol crude extract from leaves,stems and roots of Aquilegia viridiflora Pall.against Myzus persicae(Sulzer)was measured by soaking.The ethanol crude extract from the whole plant of Aquilegia viridiflora Pall.at the flowering stage was used to carry out the field efficacy test.【Results】Using 50.0 mg/mL ethanol crude extract of Aquilegia viridiflora Pall.leaves,stems and roots treated for 72 h,the mortality and adjusted mortality of Myzus persicae(Sulzer)reached more than 70.00%,and there was no significant difference between treatments(P>0.05).The toxicity regression equation was y=2.48+1.78x(R^(2)=0.99)for leaves,y=4.84+0.47x(R^(2)=1.00)for stems and y=3.03+1.91x(R^(2)=0.91)for roots.After applying 25.0 mg/mL ethanol crude extract of whole Aquilegia viridiflora Pall.plant in the field for 7 d,the control effect of Myzus persicae(Sulzer)reached 70.31%.【Conclusions】At the blooming stage,the ethanol crude extract of Aquilegia viridiflora Pall.leaves,stems and roots had the same contact toxicity effect on Myzus persicae(Sulzer).The insecticidal active substances were evenly distributed in the whole plant.The ethanol crude extract of Aquilegia viridiflora Pall.at blooming stage had good field control effect to Myzus persicae(Sulzer).