滤片捕集-气相色谱法测定加热卷烟气溶胶中水分的含量

Determination of Moisture in Aerosol of Heated Not Burned Cigarette by Gas Chromatography with Filters Trap

基于加热卷烟抽吸前后水分转移规律,分析了环境条件、抽吸参数和滤片捕集能力等对加热卷烟气溶胶中水分测定值的影响,提出了滤片捕集-气相色谱法(GC)测定加热卷烟气溶胶中水分含量的方法。利用吸烟机,按照抽吸容量35 mL,抽吸间隔30 s,每支卷烟抽吸7口的条件对加热卷烟进行抽吸;抽吸结束后,取出一张剑桥滤片(直径44 mm),捕集加热卷烟气溶胶,每个滤片捕集5支卷烟;再用四分之一块的新剑桥滤片擦拭捕集器,将所有使用过的滤片放入100 mL的圆底瓶中,迅速加入20 mL萃取剂(由1 000.00 mL异丙醇和5.00 mL无水乙醇混合而成),密封,以150次·min^(-1)的速率振荡萃取30 min,得到的萃取液进行GC测定。结果显示:水的质量浓度在8.0 g·L^(-1)以内与其对应的峰面积与内标(乙醇)峰面积的比值呈线性关系,检出限(3S/N)为0.154 mg·支^(-1);对实际样品进行加标回收试验,回收率为98.4%~101%。方法用于实际加热卷烟分析,气溶胶中水分测定值为10.71~22.50 mg·支^(-1),测定值的相对标准偏差(n=5)均小于2.0%。

Based on the principle of moisture conservation in the heated not burn cigarettes before and after smoking,the effects of environmental conditions,puff parameters and filter capacity on the determined values of moisture in aerosol of heated not burned cigarette were analyzed,then a method for the determination of the moisture in aerosol of heated not burned cigarette by gas chromatography(GC)with filters trap was proposed.Heated not burned cigarettes were smoked by suction machine at the conditions of puff capacity of 35 mL,puff interval of 30 s and each cigarette puff number of 7.After smoking,Cambridge filter(diameter of 44 mm)was used to capture the aerosol of heated not burned cigarettes,and each filter captured 5 cigarettes.The quarter of new Cambridge filter was used to wipe the trap machine.Then the all filters used were put into a 100 mL round-bottom bottle and 20 mL of extraction agent(1000.00 mL isopropyl alcohol mixed with 5.00 mL anhydrous ethanol)were quickly added.Then the above solution was sealed and vibrating extracted at a rate of 150 times·min^(-1) for 30 min.The extraction solution was determined by GC.As shown by the results,linear relationship between value of mass concentration of moisture and ratios of its peak area to internal standard(ethanol)peak area was kept within 8.0 g·L^(-1),with detection limit(3 S/N)of 0.154 mg·cig^(-1).Test for recovery was made on actual sample at 3 concentration levels by standard addition method,giving results in the range of 98.4%^(-1)01%.This method has been used to analyze the heated not burned cigarettes.The determined values of moisture in aerosol of heated not burned cigarettes were in the range of 10.71-22.50 mg·cig^(-1),and RSDs(n=5)of the determined values were less than 2.0%.