二烯丙基二硫通过miR-7下调XIAP抑制人胃癌SGC7901细胞增殖与迁移侵袭

Diallyl disulfide downregulates XIAP through miR-7 to inhibit proliferation,migration and invasion of human gastric cancer SGC7901 cells

目的探讨二烯丙基二硫(diallyl disulfide,DADS)是否通过miR-7下调X连锁凋亡抑制蛋白(X-linked inhibitor of apoptosis protein,XIAP),抑制胃癌SGC7901细胞增殖、迁移和侵袭。方法构建XIAP过表达SGC7901细胞株;qRT-PCR、Western blot检测DADS对过表达细胞XIAP表达的影响;CCK-8和平板克隆形成实验分析DADS对XIAP过表达细胞增殖的影响;划痕和Transwell实验分析DADS对XIAP过表达细胞迁移和侵袭的影响;qRT-PCR检测DADS对miR-7表达的影响;双荧光素酶报告实验、qRT-PCR和Western blot验证miR-7对XIAP靶向负调控作用。结果XIAP过表达增强SGC7901细胞增殖、迁移和侵袭能力;DADS下调XIAP、抑制XIAP过表达细胞增殖、迁移和侵袭;DADS上调miR-7表达;miR-7靶向调控XIAP 3′UTR,过表达miR-7降低XIAP表达,而敲低miR-7则上调XIAP表达。结论DADS通过miR-7下调XIAP抑制SGC7901细胞增殖、迁移和侵袭。

Aim To investigate whether diallyl disulfide(DADS)downregulates X-linked inhibitor of apoptosis protein(XIAP)through miR-7,inhibiting the proliferation,migration and invasion of gastric cancer SGC7901 cells.Methods Gastric cancer SGC7901 cell line overexpressing XIAP was established.qRT-PCR and Western blot were used to detect the effect of DADS on XIAP expression in overexpressed cells.CCK-8 and plate clone formation assay were used to analyze the effect of DADS on the proliferation of XIAP overexpressing cells.Wound healing and Transwell experiments were employed to analyze the effects of DADS on the migration and invasion of XIAP overexpressing cells.qRT-PCR was used to detect the effect of DADS on the expression of miR-7.Negative regulation of miR-7 on XIAP was verified by using dual luciferase reporter gene assay,qRT-PCR,and Western blot.Results XIAP overexpression enhanced the proliferation,migration and invasion of SGC7901 cells.DADS downregulated XIAP and inhibited the proliferation,migration and invasion of XIAP overexpressing cells.DADS upregulated miR-7 expression.miR-7 bound and regulated XIAP 3′UTR directly.Overexpression of miR-7 decreased XIAP expression,while knockdown of miR-7 increased XIAP expression.Conclusion DADS downregulates XIAP through miR-7 to inhibit SGC7901 cell proliferation,migration and invasion.