青海亚麻籽油甘三酯指纹图谱构建及掺伪识别的研究

Triglyceride fingerprint construction and adulteration identification of Qinghai flaxseed oil

利用高效液相色谱-蒸发光散射检测器(HPLC-ELSD)法对青海亚麻籽油中的甘三酯(TAG)组分进行了定性、定量研究,并运用中药色谱指纹图谱相似度评价系统建立其TAG标准指纹图谱,利用指纹图谱鉴别掺入5%~50%的大豆油、玉米油、菜籽油、花生油、葵花籽油、芝麻油的掺伪模型。结果表明:亚麻籽油中主要的TAG为OLnLn(29.40%)、LnLnLn(23.71%)、OLnO(15.10%)、OLLn(13.43%)、LLnLn(13.32%);指纹图谱鉴别结果与真实掺伪量的相对误差表明所建立的指纹图谱可以较好地鉴别掺入5%~50%的大豆油、葵花籽油、芝麻油的掺伪模型,对花生油掺伪量10%的掺伪模型的鉴别相对误差较高(9.15%),未能实现对菜籽油掺伪量5%掺伪模型的鉴别。试验构建的青海亚麻籽油TAG指纹图谱可为青海省亚麻籽油质量监控和掺伪识别提供理论依据。

The triglyceride(TAG)components in Qinghai flaxseed oil were determined qualitatively and quantitatively by HPLC-ELSD,and the standard TAG fingerprint was established by the similarity evaluation system of traditional Chinese medicine chromatographic fingerprint.The fingerprint constructed was used to identify the adulteration model of soybean oil,corn oil,rapeseed oil,peanut oil,sunflower seed oil and sesame oil with adulteration amount 5%-50%.The results showed that the main TAGs in Qinghai flaxseed oil were OLnLn(29.40%),LnLnLn(23.71%),OLnO(15.10%),OLLn(13.43%),LLnLn(13.32%).The relative error between the identification result and the true adulteration amount showed that the fingerprint constructed could identify the adulteration models of soybean oil,sunflower seed oil and sesame oil with adulteration amount 5%-50%,and the relative error for the 10% peanut oil adulteration model was relatively high(9.15%),and the identification of the 5%rapeseed oil adulteration model could not be realized.The TAG fingerprint of Qinghai flaxseed oil constructed provides a theoretical basis for quality control and adulteration identification of Qinghai flaxseed oil.