脑卒中后抑郁症患者粪便微生物种群多样性和均衡性分析

The diversity and equilibrium of microbial populations in the stool of patients with post-stroke depression

目的分析脑卒中后抑郁症患者粪便中的微生物种群多样性和均衡性。方法选择我院2018年10月至2020年10月神经内科住院部收治的68例脑卒中后抑郁症患者为试验组。在同一时期选择我院体检中心的68例健康体检者作为对照组。采用第二代高通量测序技术对其粪便中的微生物种群多样性和均衡性进行分析。结果(1)两组对象粪便样本微生物种群Good′s Coverage指数差异无统计学意义(P>0.05),而试验组OTU种类、Chao 1指数和Ace指数高于对照组(均P<0.05)。(2)两组对象粪便样本微生物种群Shannon指数、Simpson指数和Evenness指数差异均无统计学意义(均P>0.05)。(3)两组对象粪便样本微生物种群均包括变形菌门、拟杆菌门、梭杆菌门以及厚壁菌门,其中试验组厚壁菌门相对丰度显著低于对照组,而其他3种菌门则高于对照组(均P<0.05)。(4)试验组患者粪便样本微生物种群理研菌科、氨基球菌科、梭杆菌科、Erigeronaceae、肠杆菌科和紫单胞菌科的相对丰度较高,而瘤胃菌科、普雷沃菌科、毛螺菌科以及单毒丝菌科的相对丰度较低(均P<0.05)。(5)在属水平上,试验组对象粪便样本微生物种群有11种菌属的相对丰度高于对照组,有3种菌属的相对丰度低于对照组(均P<0.05)。结论脑卒中后抑郁症患者粪便中微生物种群与健康人群存在较大差异。

Objective To analyze the diversity and balance of microbial populations in the feces of patients with post-stroke depression.Methods A total of 68 patients from the Neurology Department in our hospital from Oct 2018 to Oct 2020 were set as the observation group,with 68 healthy individuals in the physical examination center of our hospital as the control group.The second generation high-throughput sequencing was used to analyze the diversity and balance of fecal flora.Results(1)In Good's Coverage index,there was no significant difference between the two groups;the OTU type,Chao 1 index and Ace index in the observation group were higher than in the control group(all P<0.05).(2)There were no significant differences in Shannon index,Simpson index and Evenness index between the two groups.(3)The fecal flora included Proteus,Bacteroides,Clostridium and Clostridium in the two groups;the relative abundance of Phaeophyta in the observation group was significantly lower than that in the control group,while those of the other three phyla were higher than those in the control group(all P<0.05).(4)The relative abundances of Phytophagaceae,Aminococcaceae,Clostridium,Erigeronaceae,Enterobacteriaceae and Vibrionaceae in observation group were higher,but those of Ruminaceae,Prevocoiaceae,Tricunspiraceae and Monoofroiaceae were lower(all P<0.05).(5)At the genus level,the relative abundances of 11 genera were higher,while those of 3 genera were lower in the observation group than those in the control group respectively(all P<0.05).Conclusion The fecal flora of patients with post-stroke depression is quite different from that of healthy people.