类风湿关节炎外周血中miRNA-124a的表达及临床意义

Research on expression of MiRNA-124a in peripheral blood of patients with rheumatoid arthritis and it‘s clinical significance

目的通过比较类风湿关节炎(RA)患者与健康对照组中外周血单个核细胞(PBMCs)中miRNA-124a的表达差异,分析RA患者PBMCs中miRNA-124a的表达水平与血沉(ESR)、C反应蛋白(CRP)、类风湿因子(RF)、抗CCP抗体(Anti-CCP)、28个关节疾病活动性评分(DAS28)等临床指标结果是否存在相关性,为进一步探究RA的发病机制及新的治疗靶点提供新思路。方法选取初诊的80例RA患者,根据DAS28评分将RA患者分为活动组(DAS28≥2.6)和缓解组(DAS28<2.6),同期选取于我院体检的20例健康者为对照组,收集相关临床指标。采用实时定量聚合酶链式反应(RT-qPCR)方法检测miRNA-124a在RA活动组、RA缓解组及健康对照组PBMCs中的表达,分析miRNA-124a与RA临床指标的关系。结果(1)RT-qPCR验证结果显示,RA活动组PBMCs中miR-124a的表达量(0.48±0.20)低于RA缓解组(0.86±0.49),RA缓解组低于健康对照组(4.25±0.13),有显著差异(P<0.05)。(2)RA患者miRNA-124a与RA疾病活动性的相关性:RA患者PBMCs中miRNA-124a分别与ESR(r=-0.362,P<0.05),CRP(r=-0.370,P<0.05)及DAS28(r=-0.356,P<0.05)呈负相关,与RF(r=-0.145,P=0.200)、抗CCP抗体(r=-0.76,P=0.503)无显著相关性。结论(1)RA患者PBMCs中miRNA-124a的表达下调,提示miRNA在RA发病中可能起着重要作用。(2)RA患者PBMCs中miRNA-124a的表达与ESR,CRP,DAS28呈负相关关系,提示miRNA-124a有望成为评价RA疾病活动的生物标志物。

Objective In this study,the difference in the expression of miRNA-124 a in peripheral blood mononuclear cells(PBMCs)in patients with rheumatoid arthritis(RA)and those in healthy control group was compared,and whether there was a correlation between the expression of miRNA-124 a in PBMCs in RA patients with erythrocyte sedimentation rate(ESR),C-receptor protein(CRP),rheumatoid factor(RF),anti-cyclic citrullina ted peptide antibody(anti-CCP),disease activity score in 28 joints(DAS28)and other clinical indicators was analyzed,so as to provide a new idea for further exploring the pathogenesis of RA and new therapeutic targets.Methods Eighty RA patients who were newly diagnosed were selected and divided into activity group(DAS28≥2.6)and remission group(DAS28<2.6)according to DAS28 score.Besides,another 20 healthy controls during the physical examination in our hospital in the same period were selected as healthy control group.The clinical indicators of all subjects was collected and recorded.The real-time quantitative polymerase chain reaction(RT-qPCR)was applied to detect the expression of miRNA-124 a in PBMCs in RA activity group,RA remission group and healthy control group and analyze the relationship between miRNA-124 a and RA clinical indicators.Results(1)RT-qPCR verification results showed that the expression of miR-124 a in PBMCs in RA activity group(0.48±0.20)was lower than that in RA remission group(0.86±0.49),and that in RA remission group was lower than that in healthy control group(4.25±0.13),the difference was statistically significant(F=906.823,P<0.05).(2)Correlation between miRNA-124 a and RA disease activity in RA patients:miRNA-124 a in PBMCs in RA patients was negatively correlated with ESR(r=-0.362,P<0.05),CRP(r=-0.370,P<0.05)and DAS28(r=-0.356,P<0.05)and had no significant correlation with RF(r=-0.145,P=0.200)and anti-CCP(r=-0.76,P=0.503).Conclusions(1)The expression of miRNA-124 a in PBMCs in RA patients was down-regulated,which suggested that miRNA might play an important role in RA pathogenesis.(2)The expression of miRNA-124 a in PBMCs in RA patients was negatively correlated with ESR,CRP and DAS28,which suggested that miRNA-124 a was expected to become a biomarker for evaluating the disease activity of RA.