艾灸关元穴对甲状腺功能减退大鼠的影响

Effect of Moxibustion at Guanyuan Acupoint on Thyroid Disfunction of Hypothyroid Rats

目的:探讨艾灸关元穴对甲状腺功能减退大鼠甲状腺功能的影响。方法:采用随机数字表法将60只4周龄SPF级雌雄各半Wistar大鼠分为空白组、模型组和艾灸组,每组各20只,除空白组外,其余组大鼠给予0.05%丙硫氧嘧啶(propylthiouracil,PTU)喂养建立甲状腺功能减退大鼠模型。模型建立完成后,艾灸组予以关元穴艾灸,隔天1次,每次30 min,持续4个月,空白组和模型组均捆绑悬针灸,但不予以艾灸操作。采用电子天平于艾灸干预前及艾灸1个月、2个月、3个月、4个月后称量各组大鼠的体质量;ELISA法检测大鼠血清促甲状腺激素(thyroid stimulating hormone,TSH)、总三碘甲状腺原氨酸(total triiodothyroxine3,TT3)、TT4的水平;HE染色观察甲状腺组织病理变化;MTT法检测甲状腺滤泡上皮细胞增殖抑制率;流式细胞仪检测甲状腺滤泡上皮细胞凋亡率。结果:与空白组比较,模型组大鼠体质量及TT3、TT4水平显著降低(P<0.05),TSH水平及甲状腺滤泡上皮细胞增殖抑制率和凋亡率显著升高(P<0.05);与模型组比较,艾灸组大鼠体质量及TT3、TT4水平显著升高(P<0.05),TSH水平及甲状腺滤泡上皮细胞增殖抑制率和凋亡率显著下降(P<0.05)。模型组大鼠甲状腺滤泡排列不规则、呈多形性,局部增生,滤泡腔大小不均匀,滤泡上皮细胞部分呈扁平状,滤泡腔有红色深染胶质;艾灸组大鼠甲状腺滤泡及上皮细胞病理改变显著改善。结论:艾灸关元穴可明显改善甲状腺功能减退大鼠的甲状腺功能。

Objective:To investigate the effect of moxibustion at Guanyuan acupoint on thyroid function in hypothyroid rats.Methods:Sixty 4-week-old SPF Wistar rats were divided into blank group,model group and moxibustion group by random number table method,with 20 rats in each group.Except the blank group,the other groups were given 0.05%Rat model of hypothyroidism was established by feeding propylthiouracil(PTU).After the model was established,the moxibustion group was given moxibustion at Guanyuan acupoint,once every other day,for 30 min each time,for 4 months.The body weight of the rats in each group was measured by electronic balance before the moxibustion intervention and after 1 month,2 months,3 months and 4 months of moxibustion;the serum thyroid stimulating hormone(TSH),total triiodothyroxine3(TT3)and TT4 levels of the rats were detected with ELISA.HE staining was used to observe the pathological changes of thyroid tissue.MTT method was used to detect the proliferation inhibition rate of thyroid follicular epithelial cells;flow cytometry was used to detect thyroid follicles Epithelial cell apoptosis rate.Results:Compared with the blank group,the body weight and the levels of TT3 and TT4 in the model group were significantly decreased(P<0.05),while the level of TSH and the proliferation inhibition rate and apoptosis rate of thyroid follicular epithelial cells were significantly increased(P<0.05);Compared with the model group,the body weight and the levels of TT3 and TT4 in the moxibustion group were significantly increased(P<0.05),while the level of TSH and the proliferation inhibition rate and apoptosis rate of thyroid follicular epithelial cells were significantly decreased(P<0.05).The thyroid follicles of the rats in the group were irregularly arranged,pleomorphic,with local hyperplasia,the size of the follicular cavity was uneven,the follicular epithelial cells were partially flat,and the follicular cavity had red deep-stained colloid;the thyroid gland of the rats in the moxibustion group Follicular and epithelial cell pathology was significantly improved.Conclusion:Moxibustion at Guanyuan acupoint can significantly improve the thyroid function of hypothyroid rats.