Ser-AuNPs作为手性选择剂对3种肾上腺素的毛细管电泳拆分

Enantioseparation of three kinds of adrenaline by capillary electrophoresis using Ser-AuNPs as chiral selector

采用化学还原法合成了纳米金(AuNPs),并以丝氨酸(Ser)对其表面进行手性修饰合成SerAuNPs。分别采用紫外可见分光光度法和激光粒度仪对AuNPs与Ser-AuNPs的合成效果及粒径分布进行表征。进一步采用毛细管区带电泳法,以Ser-AuNPs为手性选择剂,对3种肾上腺素类药物进行手性拆分。分别考察丝氨酸络合浓度、缓冲液pH、分离电压及分离柱温等对分离效果的影响。实验结果表明,以Ser-AuNPs为手性选择剂,在一定的电泳条件下异丙肾上腺素、肾上腺素和去甲肾上腺素均能达到基线分离,分离度分别达到3.19,2.85和3.77,重现性和精密度良好。

Au nanoparticles(AuNPs)were synthesized by chemical reduction method,and its surface was chiral modified with serine to prepare Ser-AuNPs.The synthesis effect and particle size distribution of AuNPs and SerAuNPs were characterized by UV-Vis spectrophotometry and laser particle size analyzer.The Ser-AuNPs was used as a chiral selector for the enantiomeric separation of three adrenaline drugs by capillary zone electrophoresis.The influences of the binding concentration of serine,buffer pH,separation voltage and capillary temperature on the enantioseparation were investigated.The results showed that the enantiomeric separation of isoprenaline,adrenaline and noradrenaline could achieve baseline separation at the optimal separation conditions.The corresponding resolutions were 3.19,2.85 and 3.77 for isoprenaline,adrenaline and noradrenaline,respectively.And this method possessed good reproducibility and precision.