硒化修饰对白术多糖抗氧化和免疫调节作用的影响

Effects of selenylation modification on the enhancement of antioxidant capability and immune activity of Atractylodes macrocephala polysaccharide

为了考察硒化修饰对白术多糖抗氧化能力和免疫活性的影响,首先采用水提醇沉法提取白术多糖(AMP),用三氯乙酸进行纯化和柱层析洗脱,再采用HNO-NaSeO法合成了硒酸化白术多糖(sAMP),并以未修饰的AMP和亚硒酸钠为对照,以环磷酰胺致免疫功能低下的小鼠为研究对象,考察sAMP的抗氧化和免疫调节作用。结果显示,与对照组、环磷酰胺组、亚硒酸钠组和AMP组相比,sAMP组超氧化物歧化酶(SOD)水平显著升高,丙二醛(MDA)水平显著降低。sAMP促进和恢复了小鼠免疫器官的生长发育,改善了小鼠的免疫清除功能,提高了小鼠血清溶菌酶和干扰素-γ(IFN-γ)水平,降低了白细胞介素-4(IL-4)和肿瘤坏死因子-α(TNF-α)水平,提高了免疫抑制小鼠脾淋巴细胞增殖活性。结果表明:硒化修饰提高了AMP的免疫活性和抗氧化能力。

This study aims to assess the effects of selenylation modification on the enhancement of antioxidant capability and immune activity of Atractylodes macrocephala polysaccharide(AMP).AMP was extracted by water decoction and ethanol precipitation;then the purification was carried out by trichloroacetic acid to elute the protein by column chromatography.The selenizing AMP(sAMP) was synthesized using the HNO-NaSeOmethod,and unmodified AMP and sodium selenite was used as a control,and immunocompromised mice prepared by cyclophosphamide were used as the research objects to investigate the antioxidant and immunomodulatory effects of sAMP.The results demonstrated that in comparison to the control,cyclophosphamide,sodium selenite,and AMP groups,the superoxide dismutase(SOD) level was significantly elevated,while serum malondialdehyde(MDA) level was markedly reduced in the sAMP group.Furthermore,sAMP promoted and restored the growth and development of immune organs in mouse,improved the immune clearance function of mice,elevated the serum levels of lysozyme and interferon-gamma(IFN-γ),reduced the levels of interleukin-4(IL-4) and tumor necrosis factor-α(TNF-α),and increased the proliferation activity of spleen lymphocytes in immunosuppressive mice.These results indicate that selenylation modification enhanced the immune activity and antioxidant capability of AMP.