布鲁氏菌Omp2a-BtpB多表位疫苗结构预测及免疫模拟

Structure prediction and immune simulation of Brucella Omp2a-BtpB multi-epitope vaccine

目的运用生物信息学方法对布鲁氏菌Omp2a和BtpB的T、B细胞优势表位进行预测、分析,从而构建多表位疫苗。方法从UniProt数据库中获取Omp2a和BtpB的氨基酸序列,应用I-TASSER构建三级结构。选取优势表位辅以佐剂构建多表位疫苗并进行免疫模拟。结果在Omp2a中获得2个B细胞优势表位,5个CTL细胞优势表位,3个Th细胞优势表位;在BtpB中获得1个B细胞优势表位,5个CTL细胞优势表位,1个Th细胞优势表位。构建的多表位疫苗为亲水性的稳定蛋白。结论基于生物信息学方法设计了新型布鲁氏菌多表位疫苗,为进一步研究相关疫苗提供理论基础。

Objective To predict and analyze T and B cell dominant epitopes of Omp2a and BtpB of Brucella by bioinformatics and construct a novel multi-epitope vaccine.Methods The amino acid sequences of the proteins,which were obtained from UniProt Database.Furthermore,the 3D structure of the proteins was builded by I-TASSER.Then being selected dominant epitopes and adjuvants to construct an multi-epitope vaccine and perform immune simulation.Results Two B cell dominant epitopes,five CTL cell dominant epitopes and three Th cell dominant epitopes were obtained in Omp2a.One B cell dominant epitope,five CTL cell dominant epitopes and one Th cell dominant epitope were obtained in BtpB.The constructed multi-epitope vaccine was a hydrophilic stable protein.Conclusion A novel Brucella multi-epitope vaccine is designed by using a bioinformatics approach,providing a theoretical basis for further research on Brucella vaccines.