lncRNA ADPGK-AS1、miR-1301-3p的表达与胃腺癌预后的关系

Relationship between the expression of lncRNA ADPGK-AS1 and miR-1301-3p and the prognosis of gastric cancer

目的研究长链非编码RNA(lncRNA)ADPGK-AS1及微小RNA(miR)-1301-3p的表达与胃腺癌预后的关系。方法选择2016年5月至2018年5月河北医科大学第二医院收治的142例胃腺癌患者。实时定量PCR检测癌组织和癌旁组织中lncRNA ADPGK-AS1、miR-1301-3p表达,并根据两者的平均值分为lncRNA ADPGK-AS1高表达组(69例)、lncRNA ADPGK-AS1低表达组(73例)、miR-1301-3p高表达组(70例)、miR-1301-3p低表达组(72例)。Pearson相关性分析癌组织中lncRNA ADPGK-AS1与miR-1301-3p表达的相关性。采用在线生物信息学软件预测lncRNA ADPGK-AS1与miR-1301-3p之间的相互结合位点。应用Kaplan-Meier生存分析不同lncRNA ADPGK-AS1、miR-1301-3p表达水平对患者生存情况的影响。结果癌组织中lncRNA ADPGK-AS1、miR-1301-3p的表达高于癌旁组织(P<0.05)。lncRNA ADPGK-AS1与miR-1301-3p呈正相关(r=0.601,P<0.01)。lncRNA ADPGK-AS1在2422~2443碱基处与miR-1301-3p存在结合位点。lncRNA ADPGK-AS1高表达组患者中位生存期为23.3个月(95%CI:15.2~26.1),低于lncRNA ADPGK-AS1低表达组的31.6个月(95%CI:22.9~34.9)(P<0.05)。miR-1301-3p高表达组患者中位生存期为24.2个月(95%CI:17.6~28.5),低于miR-1301-3p低表达组的30.5个月(95%CI:21.6~34.5)(P<0.05)。结论胃癌中lncRNA ADPGK-AS1、miR-1301-3p表达升高,两者存在结合位点,是胃癌预后预测的潜在分子标志物。

Objective To study the relationship between the expression of long non-coding RNA(lncRNA)ADPGK-AS1 and microRNA(miR)-1301-3p and the prognosis of gastric cancer.Methods One hundred and forty-two patients with gastric cancer treated in the Second Hospital of Hebei Medical University from May 2016 to May 2018 were selected.The expression levels of lncRNA ADPGK-AS1 and miR-1301-3p in cancer tissues and adjacent tissues were detected by real time quantitative PCR,according to the average value of the two groups,they were divided into lncRNA ADPGK-AS1 high expression group(69 cases),lncRNA ADPGK-AS1 low expression group(73 cases),miR-1301-3p high expression group(70 cases),and miR-1301-3p low expression group(72 cases).Pearson correlation was used to analyze the correlation between lncRNA ADPGK-AS1 and miR-1301-3p expression in cancer tissues.The binding sites between lncRNA ADPGK-AS1 and miR-1301-3p were predicted by online bioinformatics software.Kaplan-Meier survival was used to analyze the survival situation of patients with different lncRNA ADPGK-AS1 and miR-1301-3p expression levels.Results The expressions of lncRNA ADPGK-AS1 and miR-1301-3p in cancer tissues were higher than those in adjacent tissues(P<0.05).LncRNA ADPGK-AS1 was positively correlated with miR-1301-3p(r=0.601,P<0.01).LncRNA ADPGK-AS1 had a binding site with miR-1301-3p at 2422-2443 bases.The median survival time of patients in lncRNA ADPGK-AS1 high expression group was 23.3 months(95%CI:15.2-26.1),which was lower than that in lncRNA ADPGK-AS1 low expression group of 31.6 months(95%CI:22.9-34.9)(P<0.05).The median survival time of patients in miR-1301-3p high expression group was 24.2 months(95%CI:17.6-28.5),which was lower than that in miR-1301-3p low expression group of 30.5 months(95%CI:21.6-34.5)(P<0.05).Conclusion The expressions of lncRNA ADPGK-AS1 and miR-1301-3p are increased in gastric cancer.There are binding sites between lncRNA ADPGK-AS1 and miR-1301-3p,they are potential molecular markers for predicting the prognosis of gastric cancer.