YTHDF2蛋白富集早期胃癌血浆中m6A修饰28S rRNA检测分析

m6A-modified 28S rRNAs captured by YTHDF2 in plasma of early gastric cancer

目的 通过YTHDF2蛋白富集血浆中m6A修饰28S rRNA,分析m6A修饰28S rRNA水平差异在早期胃癌肿瘤标志物筛选中的潜在应用价值。方法 基于YTHDF2蛋白特异性结合m6A修饰RNA的特性,采用结合重组蛋白YTHDF2磁球富集血浆中m6A修饰28S rRNA,通过逆转录实时定量PCR(qRT-PCR)方法分析早期胃癌患者和健康者血浆样本中m6A修饰28S rRNA的水平。结果与结论 YTHDF2重组蛋白可富集m6A修饰28S rRNA,qRTPCR检测表明早期胃癌血浆中m6A修饰28S rRNA的水平高于健康者。该研究结果为筛选获得基于m6A修饰RNA分子的胃癌早期诊断标志物分子提供了基础。

Objective To enrich m6A-modified 28S rRNAs in plasma by YTHDF2 protein,and evaluate the potential value of m6A-modified 28S rRNAs levels in the screening of early gastric cancer markers. Methods Based on the characteristics of YTHDF2 protein specifically binding to m6A-modified RNA,the m6A-modified 28S rRNAs in plasma were captured by magnetic spheres combined with the recombinant protein YTHDF2. The levels of m6A-modified 28S rRNAs in the plasma samples of patients with early gastric cancer and healthy individuals were analyzed by qRT-PCR. Results and Conclusion The recombinant protein YTHDF2 can enrich m6A-modified 28S rRNAs. The results of qRT-PCR indicate that the level of m6A-modified 28S rRNAs in the plasma of patients with early gastric cancer is higher than in healthy individuals. The results suggest that the m6A-modified 28S rRNA can be a potential molecule for screening tumor markers during early diagnosis of gastric cancer.