重庆市10~12岁儿童年龄别与性别别微量元素参考值与微量元素之间相关性研究

Study on the age-and gender-specific reference intervals of trace elements and the relationship among the trace elements in children aged 10 to 12 years in Chongqing

目的探讨重庆地区10~12岁儿童微量元素参考值范围及微量元素间的相关关系。方法采取分层整群抽样方法在重庆城乡地区抽取10~12岁儿童2058例,采集静脉血,测量静脉全血微量元素水平。采用非参数参考值估计方法计算儿童微量元素参考值,并采用广义线性回归模型分析微量元素间的相关关系。结果研究结果表明钙(Ca)、锌(Zn)、镁(Mg)、铜(Cu)和铁(Fe)的总体参考值范围分别为1.35~2.13 mmol/L,56.42~107.00μmol/L,1.23~1.81 mmol/L,8.25~22.37μmol/L和6.14~9.45mmol/L。女性儿童Mg、Cu和Fe的水平均低于男性儿童(P<0.05),城市儿童Zn和Cu的水平高于农村儿童,Ca、Mg和Fe的水平低于农村儿童(P<0.01)。Ca和Cu的水平随着年龄的增加呈现降低的趋势,Zn、Mg和Fe的水平随着年龄的增加呈现增加的趋势。广义线性回归模型显示Ca、Zn、Mg、Cu和Fe五种微量元素间均存在显著的相关关系(P<0.01)。结论该研究建立了青春期儿童年龄-性别别Ca、Zn、Mg、Cu和Fe的参考值范围。此外,年龄、性别与城乡地区均对微量元素水平有显著的影响,儿童营养性疾病的防治应考虑年龄、性别及城乡地区的差异。

Objective This study aims to discuss the reference intervals of trace elements and the relationships among trace elements in children aged 10-12 years in Chongqing.Methods Stratified cluster sampling was used to include 2058 children aged 10-12 years from urban and rural areas of Chongqing.Venous blood was collected and trace element levels in whole venous blood were measured.Non-parametric reference value estimation method was used to calculate the reference value of trace elements,and generalized linear regression model was used to analyze the relationships among trace elements.Results The study showed that total reference intervals of Ca,Zn,Mg,Cu and Fe were 1.35-2.13 mmol/L,56.42-107.0μmol/L,1.23-1.81mmol/L,8.25-22.37μmol/L and 6.14-9.45 mmol/L,respectively.The levels of Mg,Cu and Fe in girls were lower than those in boys(P<0.05);the levels of Zn and Cu in urban were higher than those in rural children,and their levels of Ca,Mg and Fe were lower than those in rural children(P<0.01).The levels of Ca and Cu de-creased with the increase of age,while the levels of Zn,Mg and Fe increased with the increase of age.The generalized linear re-gression model showed that there were significant relationships among Ca,Zn,Mg,Cu and Fe(P<0.01).Conclusion The study has established the age-and gender-specific reference intervals of Ca,Zn,Mg,Cu and Fe of adolescent.Besides,age,gender and urban-rural areas have significant impact on trace element levels,which should be taken into account in the prevention and treatment of children's nutritional diseases.