补骨脂素对人非小细胞肺癌A549细胞增殖和迁移的作用及机制研究

Study on effect and mechanism of psoralen on proliferation and migration of human non-small cell lung cancer A549 cells

目的研究补骨脂素对人非小细胞肺癌A549细胞增殖、迁移的影响,并探讨其相关的分子机制。方法采用CCK-8法检测不同浓度补骨脂素孵育24 h后对A549细胞增殖的影响。将A549细胞分为对照组和补骨脂素低、高浓度组(8、32μg/mL),给药24 h后,通过细胞划痕试验检测细胞迁移能力,Western blotting和RT-PCR法检测磷脂酰肌醇-3-激酶/蛋白激酶B(PI3K/Akt)通路相关蛋白和mRNA的表达水平。结果CCK-8检测结果显示,补骨脂素(8、16、32μg/mL)对A549细胞增殖具有显著的抑制作用(P<0.05),且呈现剂量依赖趋势。与对照组比较,补骨脂素低、高浓度组细胞迁移率、p-PI3K p85、p-Akt蛋白及PI3K、Akt的mRNA水平均显著降低(P<0.05)。结论补骨脂素可抑制人非小细胞肺癌A549细胞的增殖和迁移,作用机制可能与其对PI3K/Akt通路的调控有关。

Objective To study the effect and mechanism of psoralen on proliferation and migration of human non-small cell lung cancer A549 cells.Methods CCK-8 method was used to detect the effect of different concentrations of psoralen on the proliferation of A549 cells after 24 h of culture.A549 cells were divided into control group,psoralen low concentration group and psoralen high concentration group(8,32μg/mL).After 24 h of administration,the migration ability of cells was detected by scratch test,and the expression levels of phosphoinositide 3-kinase/protein kinase B(PI3K/Akt)pathway related protein and mRNA were detected by Western blotting and RT-PCR method.Results CCK-8 test results showed that psoralen(8,16,32μg/mL)significantly inhibited the proliferation of A549 cells(P<0.05),with a dose-dependent trend.Compared with the control group,the cell migration rate,p-PI3K p85,p-Akt proteins and PI3K,Akt mRNA levels in the psoralen low and high concentration group significantly reduced(P<0.05).Conclusion Psoralen can inhibit the proliferation and migration of human non-small cell lung cancer A549 cells,and its mechanism may be related to the regulation of PI3K/Akt pathway.