基于COⅠ和16S rRNA基因的涉案鸟类残体鉴定

Identification of a Bird Remain in a Case of Destroying Wildlife Resources Based on the COⅠand 16S rRNA Genes

利用线粒体DNA细胞色素c氧化酶亚基Ⅰ基因(COⅠ)和16S核糖体RNA(16S rRNA)基因鉴定某公安机关查获的1件鸟类残体所属物种。结果表明:鸟类残体样本的COⅠ、16S rRNA基因片段与白枕鹤(Antigone vipio)对应的序列相似度均不低于98.98%,遗传距离分别为0.001~0.007和0。基于这2个基因片段构建系统发育树,显示鸟类残体样本与白枕鹤均聚为一支,鉴定该鸟类残体属白枕鹤所有。得到的COⅠ和16S rRNA基因片段可作为鉴定鹤科(Gruidae)鸟类的分子标记。

The objective of this study is to identify the species of a bird remain seized by a public security organization using mitochondrial DNA cytochrome c oxidase subunitⅠ(COⅠ)and 16S ribosomal RNA(16S rRNA)gene barcoding.The results showed that the two barcodings had similarity not less than 98.98%with those of Antigone vipio.The genetic distance was 0.001-0.007 and 0 for COⅠand 16S rRNA,respectively.The phylogenetic trees constructed with COⅠand 16S rRNA showed the sample and A.vipio clustered together.Hence,the sample should be identified as A.vipio.And the two barcodings can be used as molecular markers for identification of Gruidae.