摘要
【目的】挖掘调控桃果实成熟过程中与乙烯、生长素和脱落酸相关的重要基因。【方法】以不同发育阶段的‘晚24号’桃果实中果皮为材料,采用高通量测序技术进行转录组测序并整合桃基因组数据库,探讨成熟过程中乙烯、生长素和脱落酸代谢及信号转导相关基因的表达变化。【结果】成熟前期较第二次快速生长期前期有最多的差异基因,其中上调表达基因1184个,下调表达基因1176个。成熟前期和成熟后期注释的差异基因显著富集在植物激素信号转导通路,从中筛选出与乙烯、生长素和脱落酸信号转导相关的基因分别有18、23和11个,进一步挖掘获得与乙烯、生长素和脱落酸合成相关差异基因分别有4、11和15个。【结论】乙烯、生长素和脱落酸代谢和信号转导过程的多个基因响应桃果实的成熟过程,进一步分析和验证这些基因的功能可为阐明桃果实成熟调控的分子机制奠定理论基础。
【Objective】To explore the important genes related to ethylene,auxin and abscisic acid in regulating peach fruit ripening.【Methods】The mesocarp from the different development stages of peach‘Wan No.24’was selected as materials.To investigate the gene expression profile alterations associated with ethylene,auxin and abscisic acid metabolism and signal transduction,transcriptome sequencing was performed by high-throughput sequencing technology,and combined use the genome database of peach.【Results】There were the most differentially expressed genes in the early ripening stage of peach fruit compared with the second rapid growth stage,including 1184 up-regulated genes and 1176 down-regulated genes.These differential genes were significantly enriched in plant hormone signal transduction pathway during early and late fruit ripening.18,23 and 11 genes related to ethylene,auxin and abscisic acid signal transduction were screened out,and further excavation obtained 4,11 and 15 differential genes related to ethylene,auxin and abscisic acid synthesis,respectively.【Conclusion】Several genes involved in ethylene,auxin and abscisic acid metabolism and signal transduction process respond to the ripening process of peach fruit.Further analysis and verification of the functions of these genes can lay a theoretical foundation for elucidating the molecular mechanism of peach fruit ripening regulation.
作者
赵雨桐
王巍
穆佳琪
杨海清
刘悦萍
ZHAO Yutong;WANG Wei;MU Jiaqi;YANG Haiqing;LIU Yueping(College of Bioscience and Resource Environment/Key Laboratory of Urban Agriculture(North China),Ministry of Agriculture and Rural Affairs,Beijing University of Agriculture,Beijing 102206,China;Beijing Pinggu District People's Government Fruit Office,Beijing 101200,China)
出处
《北京农学院学报》
2022年第3期1-8,共8页
Journal of Beijing University of Agriculture
基金
北京市自然科学基金项目(6182003)。
关键词
桃
果实成熟
转录组测序
植物激素
信号转导
Prunus persica L.
fruit ripening
transcriptome sequencing
plant hormones
signal transduction