活性炭颗粒对黄芩组织培养快速繁殖的影响

Effects of activated carbon granule on rapid propagation of Scutellaria baicalensis Georgi.by tissue culture

【目的】探究活性炭颗粒对黄芩组织培养快速繁殖的影响,为提高黄芩快速繁殖效率提供新途径。【方法】以黄芩试管苗茎基组织块为外植体,以MS+6-BA 0.1 mg/L+NAA 0.1 mg/L为基本培养基,添加活性炭颗粒0.1、0.5、1.0和2.0 g/L,观察对黄芩愈伤组织诱导及再生芽发生的影响。【结果】对照组及0.1 g/L活性炭组,均不能诱导愈伤组织发生,仅促进再生苗的增殖;0.5~2.0 g/L活性炭组,试管苗生长不佳,但是80 d后开始出现愈伤组织,并不断增殖,90 d时,愈伤组织明显可见,并出现大量芽点,质量浓度超过1.0 g/L时,可100%诱导愈伤组织的发生,1.0 g/L组及2.0 g/L组无明显差异。愈伤组织呈绿色,无褐化,芽点丰富,但是不能继续生长为小苗,在转接到去掉活性炭的培养基后,可以旺盛地再生出小苗,且小苗生长高度较一致,优于对照组。【结论】活性炭颗粒抑制黄芩愈伤组织的褐化;低质量浓度活性炭颗粒(0.1 g/L)对黄芩试管苗增殖生长无明显抑制作用,也无法诱导黄芩愈伤组织发生;高质量浓度活性炭颗粒(0.5~2.0 g/L)抑制黄芩试管苗增殖生长,但达到足够时间时(超过80 d)可以诱导愈伤组织的发生和芽点的再分化,其适宜质量浓度是1.0 g/L,但是抑制芽点继续分化成长为小苗,转接继代至去掉活性炭的培养基后,该类愈伤组织可以茂盛生长出再生苗,且其再分化效率显著高于普通愈伤组织。该研究为黄芩的组织培养快速繁殖提供一条高效率途径。

【Objective】The research explored the effect of activated carbon granule on the rapid reproduction of Scutellaria baicalensis Georgi.by tissue culture,and provided a new way to improve the rapid reproduction efficiency of Scutellaria baicalensis.【Methods】The stem-based tissue blocks of seedlings as explants and MS+6-BA 0.1 mg/L+NAA 0.1 mg/L as the basic medium with different concentrations of activated carbon granule 0.1,0.5,1.0 and 2.0 g/L,the effects on callus induction and bud reproduction were observed.【Results】Neither the control group nor the 0.1 g/L activated carbon granule group could induce callus,and only promoted the reproduction of regenerated seedlings.In the 0.5-2.0 g/L groups,the tube seedlings did not grow well,callus appeared after 80 d,continued to proliferate,and was clearly visible.A large number of bud spots also appeared at 90 d on the surface of the callus.Callus induced in 100%in 1.0 g/L and 2.0 g/L groups and no difference between these 2 groups.Callus was green and no brown,with rich bud spots which could not continue to grow into seedlings,but after being transferred to the culture medium removing the activated carbon granule,the small seedlings could be regenerated vigorously and highly consistent,much better than the control.【Conclusion】Activated carbon granule can not result in the browning of Scutellaria baicalensis callus.Low concentration of activated carbon granule(0.1 g/L)did not inhibit evidently the proliferation of regeneration seedlings,and did not induce the callus formation of Scutellaria baicalensis;High concentration of activated carbon granule(0.5-2.0 g/L)inhibited the proliferation of regeneration seedlings,however,inducted the callus occurrence and bud point differentiation insufficient time(>80 d),the appropriate concentration being 1.0 g/L,but preventing the bud points continued to grow into seedlings;After subculture to medium removal of activated carbon granule,the callus could flourish regenerate seedlings,and its redifferentiation efficiency was significantly higher than that of common callus.This study provides an efficient method for rapid propagation by tissue culture in Scutellaria baicalensis.