摘要
CRISPR-based genome editing systems have been successfully and effectively used in many organisms.However,only a few studies have reported the comparison between CRISPR/Cas9 and CRISPR/Cpf1 systems in the whole-genome applications.Although many web-based toolkits are available,there is still a shortage of comprehensive,user-friendly,and plant-specific CRISPR databases and desktop software.In this study,we identified and analyzed the similarities and differences between CRISPR/Cas9 and CRISPR/Cpf1 systems by considering the abundance of proto-spacer adjacent motif(PAM)sites,the effects of GC content,optimal proto-spacer length,potential universality within the plant kingdom,PAM-rich region(PARR)inhibiting ratio,and the effects of G-quadruplex(G-Q)structures.Using this information,we built a comprehensive CRISPR database(including 138 plant genome data sources,www.grapeworld.cn/pc/index.html),which provides search tools for the identification of CRISPR editing sites in both CRISPR/Cas9 and CRISPR/Cpf1 systems.We also developed a desktop software on the basis of the Perl/Tk tool,which facilitates and improves the detection and analysis of CRISPR editing sites at the whole-genome level on Linux and/or Windows platform.Therefore,this study provides helpful data and software for easy selection and application of CRISPR-based genome editing systems in plants.
基金
supported by grants from the National Key R&D Program of China (Grant No. 2018YFD1000105)
the National Science Foundation of China (Grant No. 31772266)
the Agricultural Breeding Project of Ningxia Hui Autonomous Region, China (Grant No. NXNYYZ20150203)
the Hundred Talents of the Chinese Academy of Sciences.
