青蒿鳖甲汤合西北燥证经验方对阴虚内燥型围绝经期焦虑大鼠的影响

Effect of Qinghao Biejia Decoction Combined with Northwest Dryness Syndrome Empirical Formula on Perimenopausal Anxiety Rats with Yin Deficiency and Internal Dryness

目的:研究探讨青蒿鳖甲汤合西北燥证经验方对阴虚内燥型围绝经期大鼠焦虑行为的作用机制。方法:采用随机数字表法将雌性大鼠分为假手术组、阴虚内燥型围绝经期焦虑组(模型组)、青蒿鳖甲汤合西北燥证经验方组(中药组)、丁螺环酮片组(西药组),每组各10只。卵巢切除后连续灌胃热性中药和饲喂“五三粉”饲料,同时联合“不确定空瓶应激法”建立阴虚内燥型围绝经期焦虑大鼠模型,结合阴道脱落细胞涂片、表皮角质层含水量、体质量、高架十字迷宫试验(elevated plus-maze,EPM)进行模型验证。采用青蒿鳖甲汤合西北燥证经验方干预2周后,EPM评价焦虑状态变化,Elisa法检测各组大鼠血清性激素6项及脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)含量并进行比较。结果:与假手术组比较,模型组大鼠阴道脱落细胞涂片未见有动情周期的变化,表皮角质层含水量、体质量均显著下降(P<0.01),进入EPM开放臂次数、开放臂停留时间明显减少(P<0.05),进入闭臂次数明显增加(P<0.05);与模型组比较,中药组血清BDNF显著升高(P<0.01),孕酮(progesterone,PROG)、催乳素(prolactin,PRL)明显升高(P<0.05),进入EPM开放臂次数、开放臂停留时间明显增加(P<0.05),进入闭臂次数明显减少(P<0.05)。结论:青蒿鳖甲汤合西北燥证经验方通过调节性激素对阴虚内燥型围绝经期大鼠的焦虑行为有明显改善作用。

Objective:To study the effects of Qinghao Biejia Decoction combined with northwest dryness syndrome empirical formula on the perimenopausal anxiety rats with Yin-deficiency and internal dryness syndrome.Methods:Healthy female SD rats were randomly divided into sham-operation group,model group,Qinghao Biejia decoction combined with northwest dryness syndrome empirical formula(Chinese medicine)group and buspirone tablets group.After ovariectomy,the perimenopausal anxiety rat model of Yin-deficiency and internal dryness syndrome was established by continuous gavage of hot traditional Chinese medicine and feeding"53 powder"feed,combined with"uncertain empty bottle stress method".Vaginal exfoliated cell smears,water content of epidermal stratum corneum,body mass,and elevated plus-maze(EPM)were used for model validation.Rats in each administration group were given intragastric administration for two weeks.Six sex hormones and BDNF concentration in serum were measured.Results:Compared with the sham-operation group,in the model group,there was no change of estrous cycle in the vaginal exfoliated cell smear,the water content of epidermal stratum corneum and body mass decreased significantly(P<0.01),the number of entering EPM open arm and residence time of open arm decreased significantly(P<0.05),and the number of entering closed arm increased significantly(P<0.05).Compared with the model group,the serum BDNF,PROG and PRL in the Chinese medicine group were significantly increased(P<0.01),the number of entering the open arm and the residence time of the open arm of EPM were significantly increased(P<0.05),and the number of entering the closed arm were significantly reduced(P<0.05).Conclusion:Qinghao Biejia decoction combined with northwest dryness syndrome empirical formula can improve the perimenopausal anxiety model rats of Yin-deficiency and internal dryness syndrome through estrogen balance adjustment.