肥胖儿童血清microRNA-27a、PPAR-γ与胰岛素抵抗的相关性研究

Correlation of serum microRNA-27a and PPAR-γ expression with insulin resistance in obese children

目的探讨肥胖儿童血清microRNA-27a(miR-27a)、过氧化物酶体增殖物激活受体γ(PPAR-γ)与胰岛素抵抗(IR)的相关性。方法选取2020年1月—2021年7月唐山市人民医院104例肥胖儿童为肥胖组,另选取同期该院体检的63名健康儿童为对照组。比较两组儿童体质量指数(BMI)、空腹血糖(FPG)、空腹胰岛素(FINS)、稳态模型评估(HOMA)-IR、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、脂联素、瘦素、抵抗素、内脂素、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、miR-27a mRNA、PPAR-γmRNA。Pearson或Spearman法分析肥胖儿童血清miR-27a mRNA、PPAR-γmRNA与BMI、代谢指标、细胞因子水平的相关性。结果与对照组比较,肥胖组BMI、FINS、HOMA-IR、TC、TG、LDL-C、瘦素、抵抗素、内脂素、IL-6、TNF-α、miR-27a mRNA升高(P<0.05),HDL-C、脂联素、PPAR-γmRNA降低(P<0.05)。Pearson或Spearman法相关性分析结果显示,肥胖儿童血清miR-27a mRNA与BMI、FINS、HOMA-IR、TC、TG、LDL-C、瘦素、抵抗素、内脂素、IL-6、TNF-α呈正相关(r/r_(s)=0.607、0.362、0.466、0.435、0.542、0.450、0.546、0.558、0.437、0.633和0.559,均P<0.05),与HDL-C、脂联素、PPAR-γmRNA呈负相关(r/r_(s)=-0.686、-0.607和-0.727,均P<0.05);肥胖儿童血清PPAR-γmRNA与BMI、FINS、HOMA-IR、TC、TG、LDL-C、瘦素、抵抗素、内脂素、IL-6、TNF-α呈负相关(r=-0.513、-0.328、-0.437、-0.340、-0.434、-0.411、-0.537、-0.514、-0.428、-0.547和-0.509,均P<0.05),与HDL-C、脂联素呈正相关(r=0.599和0.527,P<0.05);肥胖儿童HOMA-IR与TC、TG、LDL-C、瘦素、抵抗素、内脂素、IL-6、TNF-α呈正相关(r/r_(s)=0.167、0.168、0.259、0.442、0.426、0.372、0.438和0.441,均P<0.05),与HDL-C、脂联素呈负相关(r=-0.240和-0.378,均P<0.05)。结论肥胖儿童血清miR-27a mRNA高表达、PPAR-γmRNA低表达,两者与IR密切相关。

Objective To investigate the correlation between serum microRNA-27a(miR-27a),peroxisome proliferator-activated receptor-γ(PPAR-γ)expression,and insulin resistance(IR)in obese children.Methods A total of 104 obese children admitted to our hospital from January 2020 to July 2021 were selected as the obese group,and 63 healthy children who came to our hospital for physical examination in the same period were selected as the control group.Body mass index(BMI),fasting plasma glucose(FPG),fasting insulin(FINS),homeostasis model assessment(HOMA)-IR,total cholesterol(TC),triglycerides(TG),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),adiponectin,leptin,resistin,visfatin,interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),miR-27a,and PPAR-γmRNA levels were compared.Pearson/Spearman correlation was used to analyze the correlation of serum miR-27a and PPAR-γmRNA levels with BMI and metabolic indicators and cytokine levels in obese children.Results Compared with the control group,BMI,FINS,HOMA-IR,TC,TG,LDL-C,leptin,resistin,visfatin,IL-6,TNF-α,and miR-27a levels were increased(P<0.05),and HDL-C,adiponectin,and PPAR-γmRNA levels were decreased in the obese group(P<0.05).Pearson/Spearman correlation analysis showed that serum miR-27a levels in obese children were positively correlated with BMI,FINS,HOMA-IR,TC,TG,LDL-C,leptin,resistin,visfatin,IL-6,and TNF-αlevels(r/r_(s)=0.607,0.362,0.466,0.435,0.542,0.450,0.546,0.558,0.437,0.633,and 0.559;all P<0.05),and negatively correlated with HDL-C,adiponectin,and PPARγmRNA levels(r/r_(s)=-0.686,-0.607,and-0.727,all P<0.05);PPAR-γmRNA levels were negatively correlated with BMI,FINS,HOMA-IR,TC,TG,LDL-C,leptin,resistin,visfatin,IL-6,and TNF-αlevels(r=-0.513,-0.328,-0.437,-0.340,-0.434,-0.411,-0.537,-0.514,-0.428,-0.547 and-0.509;all P<0.05),and positively correlated with HDL-C and adiponectin levels(r=0.599 and 0.527,all P<0.05);HOMA-IR in obese children was positively correlated with TC,TG,LDL-C,leptin,resistin,visfatin,IL-6,and TNF-αlevels(r/r_(s)=0.167,0.168,0.259,0.442,0.426,0.372,0.438 and 0.441;all P<0.05),and negatively correlated with HDL-C and adiponectin levels(r=-0.240 and-0.378,P<0.05).Conclusion High serum miR-27a expression and low PPAR-γmRNA expression in obese children are closely associated with IR.