摘要
本实验以左旋肉碱为氢键受体和9种天然的有机酸为氢键供体构成9种环境友好型天然低共熔溶剂(Natural Deep Eutectic Solvent,NADES),并以此为溶剂,在室温条件下超声辅助高效提取羊栖菜中的多糖。实验研究表明,由左旋肉碱和苹果酸合成制备的NADES-7对羊栖菜多糖具有最佳的提取效果,优化的最佳提取条件为超声40 min,料液比1∶50,NADES含水量50%,在此条件下的提取率为13.82%。此外,NADES所提取的羊栖菜多糖具有优异的抗氧化作用,0.00864 g·mL^(-1)浓度下对DPPH自由基清除率达到68.70%,对ABTS自由基的清除率达到98.89%。相关研究对羊栖菜产品的深加工及其在大健康领域中的应用具有重要的实际参考价值。
In this study,9 natural deep eutectic solvents(NADES)composing of L-carnitine and different natural organic acids were used in ultrasonic assisted extraction of polysaccharides from Sargassum fusiforme at room temperature.The experimental results showed that the NADES-7 synthesized from L-carnitine and malic acid had the best extraction effect.The optimal extraction efficiency of 13.82%was achieved under the conditions of ultrasonic 40 min,solid-liquid ratio 1∶50,NADES water content 50%.Besides,the extracted polysaccharides had excellent antioxidant activity,at the concentration of 0.00864 g·mL^(-1),the DPPH radical scavenging rate reaches 68.70%,and the ABTS radical scavenging rate reaches 98.89%.This study can provide useful guidance for deep processing of Sargassum fusiforme and its applications in the health medical field.
作者
韦华珊
刘凌雯
孔晶
费玉清
徐林
陈正件
WEI Huashan;LIU Lingwen;KONG Jing;FEI Yuqing;XU Lin;CHEN Zhengjian(Biomaterials R&D Center,Zhuhai Institute of Advanced Technology,Chinese Academy of Sciences,Zhuhai 519000,China;School of Basic Medicine,Guangdong Medical University,Dongguan 523808,China;Zhongke Xuanjia Medical Care(Zhuhai)Health Technology Co.,Ltd.,Zhuhai 519000,China;Zhuhai Xuanjia Junxing Health Industry Development Co.,Ltd.,Zhuhai 519000,China)
出处
《现代食品》
2022年第13期144-148,152,共6页
Modern Food
基金
珠海中科先进技术研究院与珠海市担杆镇联合实验室、珠海市科创局项目(ZH22036207200025PWC、ZH22017003200028PWC、ZH22017001200078PWC)。
关键词
天然低共熔溶剂
羊栖菜多糖
提取
抗氧化作用
natural deep eutectic solvent
Sargassum fusiforme polysaccharide
extraction
antioxidant activity