碱性橙Ⅱ印迹传感器的制备及其应用

Preparation and Application of Basic Orange Ⅱ Imprinted Sensor

以纳米石墨烯和纳米银作为修饰材料修饰工作电极,制备一种以碱性橙Ⅱ为模板分子,邻氨基苯酚和邻苯二胺为复合功能单体的新型快速检测碱性橙Ⅱ的印迹传感器。运用紫外光谱法选择最佳功能单体,并研究了模板分子与功能单体之间的作用形式和作用强度,采用电化学分析法优化各种制备条件,用甲醇‑0.4 mol/L NaOH水溶液(体积比2∶1)洗脱模板分子,得到分子印迹电极,并对印迹电极的分析性能进行了研究。实验结果表明,该印迹传感器对碱性橙Ⅱ具有高选择性,样品加标平均回收率为88.45%~101.40%,相对标准偏差(RSD)在1.31%~2.83%之间(n=5),线性范围为3.0×10^(-9)~5.0×10^(-5) mol/L,检出限为1.0×10^(-9) mol/L。该传感器成功应用于食品中碱性橙Ⅱ残留的快速检测。

A novel imprinted sensor for rapid detection of basic orange Ⅱ was developed by usingnano-graphene and nano-silver as modification materials to modify the working electrode with basic orange Ⅱ as template molecule,o-aminophenol and o-phenylenediamine as functional composite monomers.Theoptimal composite functional monomer was selected by ultraviolet(UV)spectroscopy,and the interactionform and intensity between the template molecule and composite functional monomer were researched by UVspectroscopy.The preparation conditions of the sensor were optimized by electrochemical analysis.Templatemolecules were eluted in methanol/0.4 mol/L Na OH solution(volume ratio 2∶1)to obtain a molecularlyimprinted electrode.The performance of the sensor was investigated.The results show that the sensor has ahigh selectivity to basic orange Ⅱ,the average recoveries of the spiked samples are between 88.45% and 101.40% with the relative standard deviations(RSDs)of 1.31%~2.83%(n=5),the linear range of 3.0×10^(-9)~5.0×10^(-5)mol/L with the detection limit of 1.0×10^(-9)mol/L.The sensor is successfully applied tothe rapid detection of basic orange Ⅱ residues in foods.