高效液相色谱法测定柴辛注射液中有效组分含量的研究

Study on Simultaneously Determination Method of Methyleugenol and Asarinin in Chaixin Injection by High Performance Liquid Chromatography

为同时测定柴辛注射液中有效组分甲基丁香酚、细辛脂素的含量,建立了以核壳型分离填料为色谱柱,乙腈和水为流动相,检测器波长为287 nm,流量为1.0 mL/min的高效液相色谱方法。结果显示,在0.15~150μg/mL的质量浓度范围内,甲基丁香酚的线性曲线为Y=7243X-258,R^(2)=0.9999,检测限为0.025μg/mL,平均回收率为97.47%~100.53%,RSD为0.92%~2􀆰17%;细辛脂素的线性曲线为Y=18853X-676,R^(2)=0.9999,检测限为0.012μg/mL,平均回收率为88.47%~92.23%,RSD为1.48%~3.55%。该方法操作简单,准确度、重复性、精密度好,可适用于柴辛注射液的质量控制。

In order to determine the methyleugenol and asarinin in Chaixin Injcetion,a high performance liquid chromatography(HPLC)method was established.Under the optimized conditions,the analyte was carried out with acetonitrile-water as a mobile phase at a flow rate of 1.0 mL/min and a wavelength of 287 nm on a coreshell column.The results showed good linear relationships of methyleugenol and asarinin within the mass concentrations of 0.15 to 150μg/mL and the correlation coefficients were all more than 0.9999.The detection limits of methyleugenol and asarinin were 0.025μg/mL and 0.012μg/mL,while the average recoveries of them were between 88.47%and 100.53%,with the relative standard deviations between 0.92%and 3.55%.The purposed method is simple to operate with good accuracy,repeatability,precision,which is suitable to the quality control.