针刺对血管性认知障碍大鼠学习记忆能力和海马Ngb表达的影响

Effect of acupuncture on learning and memory ability and hippocampal Ngb expression in rats with vascular cognitive impairment

目的探讨针刺对血管性认知障碍(VCI)模型大鼠学习记忆能力及海马区脑红蛋白(neuroglobin,Ngb)表达的影响。方法采用双侧颈总动脉反复结扎以引起缺血再灌注损伤法制备大鼠VCI模型。将造模成功大鼠按随机数字表法分为针刺组和模型组,每组12只,选取12只大鼠作为正常组。针刺组大鼠针刺"百会""风府""涌泉",10次为1个疗程,共3个疗程,疗程间停止针刺2 d。干预结束后第3天进行神经行为学评分,采用Morris水迷宫法检测各组大鼠学习记忆能力,采用Western blot法检测大鼠海马组织Ngb表达。结果与模型组比较,针刺组干预后行为学评分降低(P<0.05),逃避潜伏期明显缩短(P<0.05),穿越平台次数增多(P<0.05),海马Ngb[(1.18±0.06)比(0.98±0.04)]表达升高(P<0.05)。结论针刺"百会""风府""涌泉"可促进VCI模型大鼠海马区Ngb表达,改善其学习和记忆能力。

Objective To discuss the effect of acupuncture on learning and memory ability and the expression of neuroglobin(Ngb)of the hippocampus of vascular cognitive impaired(VCI)rats.Methods VCI rats model was established by repeated ligation of the carotid arteries of the two sides to induce ischemia-reperfusion injury.The successful modeled rats were divided into acupuncture group and a model group according to the random number table method,with 12 rats in each group,and 12 rats were selected as the normal group.The rats in the acupuncture group received acupuncture at"Baihui","Fengfu"and"Yongquan",10 times as a course of treatment,a total of 3 courses,and stop acupuncture treatment for 2 days during the courses of treatment.Neurobehavioral scores were measured on the 3rd day after the intervention.Morris water maze was conducted to detect the learning and memory ability of the rats in each group,and Western blot was used to detect the expression of Ngb in the hippocampus of the rats.Results Compared with the model group,the behavioral score of the acupuncture group decreased after intervention(P<0.05),the escape latency was significantly shortened(P<0.05),the number of crossing platforms was increased(P<0.05),and the hippocampal Ngb(1.18±0.06 vs.0.98±0.04)was significantly increased(P<0.05).Conclusion Acupuncture at"Baihui","Fengfu"and"Yongquan"could promote the expression of Ngb in the hippocampus of rats and improve the learning and memory ability of VCI rats.