基于网络药理学探讨养阴益气合剂在肺炎恢复期的作用机制

Study on the mechanism of Yangyin Yiqi mixture in the recovery period of pneumonia based on network pharmacology

目的探讨院内制剂养阴益气合剂对不同类型肺炎恢复期的作用机制,以期为肺炎的中药防治提供更多的临床选择。方法基于异病同治理论,借助中医药整合药理学研究平台(TCMIP)V 2.0,对养阴益气合剂中药有效成分、作用靶点、疾病靶点进行筛选,利用Cytoscape软件构建"疾病-靶点-方药"网络,对关键靶点进行Reactome富集分析。结果确立了7个不同类型肺炎恢复期临床表现,包括乏力(Fatigue)、气短(Tachypnea)、口渴(Xerostomia)、心悸(Palpitations)、汗多(Hyperhidrosis)、纳差(Poor Appetite)、咳嗽(Cough)。获得养阴益气合剂全方化学成分276个,"疾病-方药"关键核心靶点177个,药物与疾病共有靶点37个,关键靶点与细胞代谢、细胞凋亡、炎症反应及免疫应答、应激反应等相关。合剂中黄精与肺炎恢复期的7个病症有17个共有靶点。Reactome富集分析显示,养阴益气合剂治疗不同类型肺炎恢复期主要症状的核心基因参与的通路主要富集在:通过调控基因与蛋白质翻译,影响病毒或细胞功能;机体免疫调节及炎症反应;调节机体神经系统功能;防止病毒感染型癌变。结论养阴益气合剂有效成分可能通过干预PIK3CA、MAPK3、BCL2、NFκB2等靶点调节PI3K、IL-4、IL-13、抑制性卡巴蛋白(IκBα)表达,促进肺炎的恢复。本研究可为不同类型肺炎恢复期的中药防治提供理论依据。

Objective To study the mechanism of Yangyin Yiqi mixture on the clinical symptoms in convalescent period of different types of pneumonia based on network pharmacology,so as to provide more options for the prevention and treatment of pneumonia with Traditional Chinese Medicine(TCM).Methods Based on the TCM theory of"treating different diseases with the same method",the Integrative Pharmacology-Based Research Platform of Traditional Chinese Medicine(TCMIP)V 2.0 was used to screen the active components,action targets and disease targets of the herbs in Yangyin Yiqi mixture.The disease-target-medicine network map was constructed by Cytoscape software and the Reactome enrichment analysis of key targets were carried out.Results Seven different types of clinical sympotms of pneumonia in convalescent period were established,including fatigue,tachypnea,thirst,palpitations,hyperhidrosis,poor appetite and cough.After the screening,a total of 276 active components and 177 key targets were obtained,37 common targets of this herb and pneumonia were obtained.The key targets were associated with cell metabolism,apoptosis,inflammatory response,immune response,and stress response,etc.There are 17 common targets between Polygonatum and 7 clinical symptoms in the recovery period of different types of pneumonia.Reactome enrichment analysis showed that the pathways involved in the core genes of Yangyin Yiqi mixture in the treatment of the main symptoms of different types of pneumonia in the recovery period were mainly enriched in:by regulating genes and protein translation to affect the virus or cell function,immune regulation and inflammatory response and regulate the function of nervous system,and prevent viral canceration.Conclusions The effective components in Yangyin Yiqi mixture may regulate phosphatidylinositol 3-kinase(PI3K),interleukin-4,interleukin-13(IL-4,IL-13)and inhibitory kappa protein(IκBα)by intervention of PIK3CA,MAPK3,BCL2 and NF-κB2 and other targets to promote the recovery of pneumonia.This study could provide a theoretical basis for the prevention and treatment of pneumonia in the recovery period.