miR-30b在三叉神经痛模型大鼠中的表达及作用

Expression and effect of miR-30b in the trigeminal neuralgia model rats

目的探讨miR-30b在三叉神经痛(TN)模型大鼠三叉神经节(TG)中发挥的作用。方法采用眶下神经缩窄术(ION-CCI)构建大鼠TN模型。用Von Frey毛刷检测大鼠的机械痛阈。通过qRT-PCR及Western blot检测术侧TG内miR-30b和激活转录因子3(ATF3)表达变化。经眶下孔分别向ION-CCI大鼠的TG内定位注射miR-30b的类似物(agomir)和agomir的阴性对照(agomir NC),检测大鼠的机械痛阈及干预后ATF3表达的变化。结果ION-CCI组大鼠在术后第2~28天出现机械痛阈的降低(P<0.05)。ION-CCI组较假手术组(Sham组)术后TG中miR-30b表达降低,ATF3表达升高(P<0.05)。ION-CCI组过表达miR-30b后,大鼠机械痛阈升高,TG中ATF3表达降低(P<0.05)。结论miR-30b参与调控TN的发生、发展,miR-30b可能是TN潜在的治疗靶点。

Objective To explore the role of miR-30b in the trigeminal ganglion( TG) of trigeminal neuralgia( TN) model rats. Methods An infraorbital nerve-chronic constriction injury( ION-CCI) model of TN was produced in the rat. Von Frey hair was used to determine the time-course of changes in mechanical pain threshold.qRT-PCR and Western blot were used to detect the expression of miR-30b and activating transcription factor 3( ATF3) in the TG. In addition,miR-30b agomir and agomir NC were injected into ION-CCI rats by the infraorbital foramen injection. The mechanical pain threshold and the expression changes of ATF3 were determined after intervention. Results Compared with the sham-operated group,the ION-CCI group had a decreased mechanical pain threshold 2 days after surgery,reached the lowest observed level by day 12,and this threshold reduction lasted more than 28 days( P < 0. 05). Compared with the sham-operated group,the ION-CCI group had a lower miR-30b expression and a higher ATF3 expression in postoperative TG( P < 0. 05). After the overexpression of miR-30b in ION-CCI group,the mechanical pain threshold increased,and the expression of ATF3 decreased in TG( P <0. 05). Conclusion These results suggest that miR-30b may participate in the regulation of TN. miR-30b may be a potential therapeutic target for TN.