HB-EGF和uPAR在子痫前期中发病的联系

Application of HB-EGF and uPAR in preeclampsia

目的探究肝素结合型表皮生长因子(HB-EGF)和尿激酶型纤溶酶原激活剂受体(uPAR)与子痫前期的发病是否相关。方法首先通过抗体芯片技术筛选差异表达蛋白,检索大量文献,从中选取uPAR及HB-EGF进行验证。收集30例正常孕妇和50例子痫前期孕妇的血清标本,用ELISA方法测定血清中HB-EGF、uPAR的表达,再选取10例子痫前期孕妇和6例正常孕妇的胎盘组织进行qRT-PCR及免疫组化等方法来进行进一步验证。结果子痫前期孕妇血清uPAR的表达高于正常孕妇(P<0.05),血清HB-EGF的平均水平低于正常孕妇(P<0.05)。qRT-PCR结果显示胎盘中uPAR mRNA表达量较低甚至不表达,HB-EGF mRNA的子痫前期孕妇的表达水平低于正常孕妇(P<0.05)。免疫组织化学显示uPAR及HB-EGF在子痫前期孕妇胎盘中的表达均低于正常孕妇。结论HB-EGF及uPAR分子的异常表达与子痫前期有关。此研究证明HB-EGF和uPAR与子痫前期的发病相关,同时血液中HB-EGF和uPAR的表达差异可能对寻找生物标志物早期诊断有着指导作用。

Objective The main purpose of this study is to explore the relationship between hepafin-binding epidermal growth factor-like growth factor(HB-EGF) and urokinase-type plasminogen activator receptor(uPAR) with the pathogenesis of preeclampsia. Methods Firstly, the differentially expressed proteins were screened by antibody chip technology, a large number of literatures were searched, and uPAR and HB-EGF were selected for verification. Serum samples from 30 normal pregnant women and 50 preeclampsia pregnant women were collected, and the expressions of HB-EGF and uPAR in serum were determined by ELISA. Placental tissues of 10 preeclampsia pregnant women and 6 normal pregnant women were selected for further validation by qRT-PCR and immunohistochemistry. Results The expression of u PAR in serum in preeclampsia pregnant women was higher than that in normal pregnant women( P < 0. 05),and the average level of HB-EGF in serum was lower than that of normal pregnant women( P < 0. 05). The results of qRT-PCR showed that the expression level of u PAR mRNA in placenta was low or no,and the expression level of HB-EGF mRNA in preeclampsia pregnant was lower than that in normal pregnant women( P < 0. 05). Immunohistochemistry showed that the expression of u PAR and HB-EGF in the placenta of preeclampsia pregnant was lower than that of normal pregnant women. Conclusion The abnormal expression of HBEGF and u PAR is related to preeclampsia. This study proves that HB-EGF and u PAR are related to the pathogenesis of preeclampsia,and the difference in the expression of HB-EGF and u PAR in blood may play a guiding role in the early diagnosis of biomarkers.