酿酒酵母表面展示金属硫蛋白吸附葡萄酒中的铜离子

The Adsorption of Copper Ions in Wine by Displaying Metallothionein on the Surface of Saccharomyces cerevisiae

在葡萄酒酿造过程中,过量的铜会抑制发酵,导致葡萄酒发生氧化褐变。金属硫蛋白是一类半胱氨酸含量高,分子质量低,可被金属诱导表达的蛋白质。利用细胞表面展示技术来展示金属硫蛋白,可在很大程度上规避金属离子跨膜的问题,降低重金属对细胞造成的损伤。本研究将来自于酿酒酵母288c菌株的金属硫蛋白基因CUP1在酿酒酵母细胞表面展示,成功获得1株对葡萄酒中铜离子具有较高吸附力的二倍体菌株。为解决葡萄酒中铜破败问题提供新思路,为实现CUP1金属硫蛋白全细胞吸附剂的工业化应用奠定理论基础。

During the winemaking process,excessive copper has the effect of inhibiting fermentation,causing oxidative browning of wine.Metallothionein is a type of protein with high cysteine content and low molecular weight that can be induced by metals.The use of cell surface display technology to express that metallothionein can largely avoid the problem of metal ions transmembrane and reduce the damage of heavy metals to cells.In this study,the metallothionein gene CUP1 from Saccharomyces cerevisiae 288c was displayed on the surface of S.cerevisiae cells,and a diploid strain with higher adsorption capacity for copper ions in wine was successfully obtained.The research can provide new ideas for solving the problem of copper in wine and lay a theoretical foundation for the industrial application of CUP1 metallothionein whole-cell adsorbent.