枸杞甘草配制酒对小鼠的免疫调节和体内抗氧化作用

Immunomodulatory and Antioxidant Activity Effect of Lycium barbarum-Glycyrrhiza Liqueur on Mice

目的:研究枸杞甘草配制酒对免疫抑制KM雄性小鼠的免疫调节和体内抗氧化作用。方法:通过构建免疫抑制KM雄性小鼠模型,将小鼠随机分为6组,即空白对照组(CK)、环磷酰胺模型组(CY)、基酒对照组及枸杞甘草配制酒低、中、高剂量组,分别经口给予生理盐水或5,10,20 mL/kg bw枸杞甘草配制酒,连续灌胃14 d。末次注射后测定各组动物体质量、免疫器官脏器指数、吞噬指数;测定各组小鼠免疫球蛋白G(IgG)与免疫球蛋白M(IgM)、白细胞介素-6(IL-6)、干扰素-γ(IFN-γ)、肿瘤坏死因子-α(IFN-α)含量;测定各组小鼠谷胱甘肽过氧化物酶(GSH-Px)活力、超氧化物歧化酶(SOD)活力、丙二醛(MDA)浓度。结果:与模型组相比,枸杞甘草配制酒中剂量组能显著改变Ig G、Ig M及细胞因子IL-6、IFN-γ、IFN-α含量及GSH-Px、SOD活力、MDA浓度。结论:枸杞甘草配制酒能有效增强由环磷酰胺所致免疫低下小鼠的免疫调节和体内抗氧化作用。

Objective:To explore the regulatory effect of Lycium barbarum-Glycyrrhiza liqueur on immune function and antioxidant status in mice.Methods:Immunosuppressed mouse model was constructed,the mice were randomly divided into 6 groups:control check(CK),cyclophosphamide model group(CY),base wine control group and high,medium and low-dose Lycium barbarum-Glycyrrhiza liqueur groups,normal saline or 20,10,5 mL/kg bw Lycium barbarum-Glycyrrhiza liqueur were given orally for 14 days.After the last injection,the animal mass,thymus index,spleen index,phagocytic index,immunoglobulin G(IgG)and immunoglobulin M(IgM)content,interleukin-6(IL-6),interferon-γ(IFN-γ)and tumor necrosis factor-α(IFN-α)were determined in each group.The activity of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px),and the concentration of malondialdehyde(MDA)in serum were determined in each group.Results:compared with the model group,the contents of IgG,IgM,IL-6,IFN-γ,IFN-α,GSH Px,SOD activity and MDA concentration were significantly changed in the medium dose group.The results showed that Lycium barbarum and licorice wine could effectively improve the immune regulation and antioxidation of immunosuppressive mice induced by cyclophosphamide.