摘要
为了解蓝莓果实表面真菌菌群多样性,明确不同品种蓝莓表面的优势菌群,采用高通量测序技术分析北陆、蓝金、莱克西、奥尼尔蓝莓中ITS1区基因序列,比较不同品种蓝莓表面微生物群落结构组成及多样性差异。结果发现:蓝莓主要的优势属为枝孢属、链格孢属;优势种为枝孢菌、链格孢、枝孢霉。由Simpson指数可知微生物群落丰度及群落多样性由高至低为莱克西、北陆、奥尼尔、蓝金。不同品种蓝莓表面的菌群结构与品种存在一定的聚类关系:北陆和莱克西群落结构相似度较高,而其它样品间微生物群落差异较大,各品种蓝莓表面的主要菌落相对丰度差异较大,枝孢菌、链格孢、枝孢霉为主要差异菌种。研究初步明确了蓝莓表面菌群结构的多样性,为保证蓝莓贮藏期间的品质安全提供一定的理论支持。
In order to understand the diversity of fungal flora in different varieties of blueberries and clarify their respective dominant bacterial groups,this paper used high-throughput sequencing technology to analyze the gene sequences of ITS1 region in Hokuriku,Lanjin,Lexie,O'Neill blueberries,and compared the differences in microbial community structure and diversity from the surface of different blueberries.The results showed that the main dominant genus of blueberry were Cladosporium and Alternaria;and the dominant species were Cladosporium_chasmanthicola,Alternaria_alternata,Cladosporium_cladosporioides.The Simpson index showed that the abundance and diversity of the microbial community from high to low were Lexie,Hokuriku,O'Neill,and Blue Gold.There was a certain clustering relationship between the microbial community structure and varieties on the surface of different blueberries:Hokuriku and Lexie community structures were relatively similar,while the microbial communities of the other samples were quite different,and the relative abundance of the main colonies was significantly different,Cladosporium,Alternaria,and Cladosporium were the main different species.This study preliminarily defined the diversity of fungal flora in different varieties of blueberries,and provided certain theoretical support for ensuring the quality and safety of blueberries during storage.
作者
曹森
董立超
张鹏
薛友林
贾晓昱
李江阔
Cao Sen;Dong Lichao;Zhang Peng;Xue Youlin;Jia Xiaoyu;Li Jiangkuo(School of Food and Pharmaceutical Engineering,Guiyang University,Guiyang 550005;College of Light Industry,Liaoning University,Shenyang 110036;Institute of Agricultural Products Preservation and Processing Technology,Tianjin Academy of Agricultural Sciences,Tianjin 300384;Tianjin Key Laboratory of Postharvest Physiology and Storage of Agricultural Products,Key Laboratory of Storage of Agricultural Products,Ministry of Agriculture and Rural Affairs,National Engineering and Technology Research Center for Preservation of Agricultural Products(Tianjin),Tianjin 300384)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2022年第7期278-286,共9页
Journal of Chinese Institute Of Food Science and Technology
基金
国家重点研发计划项目(2018YFD0401303)
天津市农业科学院青年创新项目(2019009)
贵州省科技计划项目(黔科合支撑[2021]一般122)。
关键词
高通量测序
蓝莓
菌群结构
品种
high-throughput sequencing
blueberry
fungal flora structure
variety