胶质瘤组织中lncRNA NR2F2-AS1、miR-129-5p的表达及临床意义

Expression and clinical significance of lncRNA NR2F2-AS1 and miR-129-5p in glioma

目的检测胶质瘤组织中长链非编码RNA(lncRNA)核受体亚家族2组F成员2反义核糖核酸1(NR2F2-AS1)和微小RNA-129-5p(miR-129-5p)的表达,并探讨其临床意义。方法选取2015年1月至2016年9月山西省人民医院神经外科接受手术治疗的103例胶质瘤患者的胶质瘤组织及同期因颅脑手术切除的50例正常脑组织为研究对象。采用实时荧光定量PCR(qRT-PCR)检测组织样本中lncRNA NR2F2-AS1和miR-129-5p的表达水平,分析两者的相关性,分析两者的表达变化与胶质瘤患者临床病理参数的关系,用Kaplan-Meier曲线分析lncRNA NR2F2-AS1、miR-129-5p表达水平与胶质瘤患者术后5年累积生存率的关系,Cox回归分析法分析影响胶质瘤患者预后的因素。结果胶质瘤组织中lncRNA NR2F2-AS1相对表达量高于正常脑组织,而miR-129-5p相对表达量低于正常脑组织(均P<0.05)。胶质瘤组织中lncRNA NR2F2-AS1与miR-129-5p的表达呈负相关(r=-0.756,P<0.05)。胶质瘤组织中lncRNA NR2F2-AS1与miR-129-5p的表达与肿瘤WHO分级及肿瘤长径有关(均P<0.05)。LncRNA NR2F2-AS1<2.89组患者术后5年累积生存率高于lncRNA NR2F2-AS1≥2.89组患者(P<0.05),miR-129-5p<0.55组患者术后5年累积生存率低于miR-129-5p≥0.55组患者(P<0.05)。LncRNA NR2F2-AS1高表达、miR-129-5p低表达、WHO分级Ⅲ~Ⅳ级及肿瘤长径是影响胶质瘤患者术后预后的危险因素(均P<0.05)。结论胶质瘤组织中lncRNA NR2F2-AS1表达升高、miR-129-5p表达降低,两者参与脑胶质瘤临床生物学进展,并与患者预后密切相关。

Objective To detect the expression level of long non-coding RNA(lncRNA)nuclear receptor subfamily 2 group F member 2-antisense RNA 1(NR2F2-AS1)and microRNA-129-5p(miR-129-5p)in glioma tissues and to explore its clinical significance.Methods The glioma tissues of 103 patients with glioma who underwent surgical treatment in the neurosurgery department of Shanxi Provincial People′s Hospital from January 2015 to September 2016 and 50 normal brain tissues removed due to craniocerebral surgery in the same period were selected as the research objects.Real time fluorescence quantitative polymerase chain reaction(qRT-PCR)was used to detect the expression levels of lncRNA NR2F2-AS1 and miR-129-5p in tissue samples.The correlation between the two indexes and the relationship between the expression changes of the two and clinicopathological parameters of glioma patients were analyzed.Kaplan Meier curve was used to analyze the 5-year cumulative survival rate of glioma patients with different expression levels of lncRNA NR2F2-AS1 and miR-129-5p.Cox regression analysis was used to analyze the factors affecting the poor prognosis of glioma patients.Results Compared with normal brain tissue,the relative expression of lncRNA NR2F2-AS1 in glioma was higher and the relative expression of miR-129-5p was lower(all P<0.05).There was significant negative relationship between the relative expression of lncRNA NR2F2-AS1 and miR-129-5p in glioma(r=-0.756,P<0.05).The expression of lncRNA NR2F2-AS1 and miR-129-5p in glioma was related to World Health Organization(WHO)grade and tumor length(all P<0.05).The 5-year cumulative survival rate of patients in lncRNA NR2F2-AS1<2.89 group was higher than that in lncRNA NR2F2-AS1≥2.89 group,and the 5-year cumulative survival rate of patients in miR-129-5p<0.55 group was lower than that in miR-129-5p≥0.55 group(all P<0.05).Multivariate analysis showed that high expression of lncRNA NR2F2-AS1,low expression of miR-129-5p,WHO gradeⅢ-Ⅳand tumor length were the risk factors affecting the prognosis of patients with glioma(all P<0.05).Conclusions The increased expression of lncRNA NR2F2-AS1 and the decreased expression of miR-129-5p in glioma tissues are involved in the clinical biological progress of glioma and are closely related to the prognosis of patients.