CAB39、VEGF在膀胱癌中的表达及对患者预后的影响

Expression of calcium-binding protein 39 and vascular endothelial growth factor in bladder cancer and their effect on prognosis

目的探讨膀胱癌组织中钙结合蛋白39(CAB39)、血管内皮生长因子(VEGF)的表达意义及其与患者预后的关系。方法采用病例对照研究方法,选取淄博市中心医院2014年6月至2018年4月经手术获取的94例膀胱癌患者的病灶组织(病例组)及其癌旁组织(对照组),采用蛋白免疫印迹(Western blot)法、免疫组化染色技术检测两种标本中的VEGF、CAB39蛋白表达,分析不同病理学特征的膀胱癌组织中的VEGF、CAB39蛋白阳性表达率差异,采用Cox比例风险回归模型分析VEGF、CAB39蛋白表达与膀胱癌患者预后的关系。结果病例组的VEGF、CAB39蛋白相对表达量均显著高于对照组,差异均有统计学意义(均P<0.05);病例组的VEGF、CAB39蛋白的免疫组化染色评分均高于对照组,差异均有统计意义(均P<0.05)。在低级别、肿瘤临床分期T3~T4、有淋巴结转移的膀胱癌患者癌组织中CAB39蛋白阳性表达率明显更高,差异均有统计学意义(均P<0.05);在肿瘤临床分期T3~T4、有淋巴结转移的膀胱癌患者癌组织中VEGF蛋白阳性表达率明显更高,差异均有统计学意义(均P<0.05);Cox比例风险回归模型结果显示,肿瘤低级别、临床分期T3~T4、有淋巴结转移、VEGF蛋白阳性表达、CAB39蛋白阳性表达是膀胱癌患者3年不良预后结局的独立危险因素(均P<0.05)。结论VEGF、CAB39蛋白在膀胱癌组织中呈高表达,并且与膀胱癌患者的不良预后具有一定的关系。

Objective To explore the expression of calcium-binding protein 39(CAB39)and vascular endothelial growth factor(VEGF)in bladder cancer tissues and their relationship with the prognosis of patients.Methods A case-control study was conducted to select the focal tissues(case group)and post-operative paracancourous tissues(control group)from 94 bladder cancer patients who underwent surgery from Zibo Central Hospital from June 2014 to April 2018.Western blot and immunohistochemical staining were used to detect the expression of VEGF and CAB39 proteins in the two specimens.The positive expression rates of VEGF and CAB39 protein in bladder cancer tissues with different pathological characteristics were analyzed,and the relationship between VEGF and CAB39 protein expression and prognosis of patients with bladder cancer was analyzed by Cox proportional hazard regression model.Results The relative expression levels of VEGF and CAB39 protein in the case group were significantly higher than those in the control group,with significant statistical difference(all P<0.05).The immunohistochemical staining scores of VEGF and CAB39 protein in the case group were higher than those in the control group,with statistically significant difference(all P<0.05).The positive expression rate of CAB39 protein was significantly higher in bladder cancer tissure of patients with low-grade,clinical stage T3-T4 and lymph node metastasis,with statistically significant difference(all P<0.05).The positive expression rate of VEGF protein was significantly higher in bladder cancer tissue of patients with clinical stage T3-T4 and lymph node metastasis,with statistically significant difference(all P<0.05).Cox proportional hazard regression model showed that low grade,clinical stage T3-T4,lymph node metastasis,positive expression of VEGF and CAB39 protein were independent risk factors for poor prognosis in bladder cancer patients in 3 years(all P<0.05).Conclusions VEGF and CAB39 proteins are highly expressed in bladder cancer tissue,and have a certain relationship with the poor prognosis of patients.