HPLC波长切换法测定不同产地紫菀中芹菜素、槲皮素和紫菀酮的含量

Determination of Apigenin,Quercetin and Asterone in Aster from Different Places by HPLC Wavelength Switching

建立HPLC波长切换法同时测定紫菀中芹菜素、槲皮素和紫菀酮的含量。色谱柱为Agilent ZORBAX SB-C18(4.6×250mm,5μm)柱;流动相:乙腈-0.1%磷酸溶液,梯度洗脱;柱温40℃;流速1.0mL/min;检测波长:0~30min设为360nm(测定槲皮素和芹菜素),30~55min设为200nm(测定紫菀酮)。结果表明,芹菜素、槲皮素和紫菀酮分别在8.23~131.71μg/mL(r=0.9999)、11.08~177.29μg/mL(r=0.9998)、12.58~201.29μg/mL(r=1.000)范围内线性关系良好,平均回收率分别为99.53%(RSD=1.51%)、99.38%(RSD=1.68%)、99.75%(RSD=1.74%)。

Objective To establish a wavelength-switching HPLC method for the simultaneous determination of apigenin,quercetin and asterone in Aster.Methods The chromatographic column was an Agilent ZORBAX SBC18(4.6×250mm,5μm)column;mobile phase:acetonitrile-0.1%phosphoric acid solution,gradient elution;column temperature 40℃;flow rate 1.0mL/min;detection wavelength:0~30min was set to 360nm(determination of quercetin and apigenin),and 30 to 55 minutes was set to 200nm(determination of asterone).Results Apigenin,quercetin and asterone were linear in the range of 8.23-131.71μg/mL(r=0.9999),11.08-177.29μg/mL(r=0.9998),12.58-201.29μg/mL(r=1.000),respectively The relationship was good,and the average recovery rates were 99.53%(RSD=1.51%),99.38%(RSD=1.68%),and 99.75%(RSD=1.74%).